Proteomic analysis using protein chips to detect biomarkers in cervical and amniotic fluid in women with intra-amniotic inflammation

• Intra-amniotic inflammation (IAI) may cause preterm birth with poor neonatal out-come. To identify novel biomarkers for IAI, we analyzed amniotic and cervical fluid samples from 27 patients with signs of threatening preterm birth with or without IAI by surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS). Seventeen proteins were significantly overexpressed in amniotic fluid from IAI cases and more often in women with preterm labor than those with rupture of membranes. Five of these were identified as human neutrophil protein 1-3, calgranulin A and B.

Nyckelord

Amniocentesis

Amnion/metabolism

Amniotic Fluid/chemistry/*metabolism

Biological Markers/*chemistry

Calgranulin A/metabolism

Calgranulin B/metabolism

Cervix Uteri/metabolism

Female

Fetal Membranes

Premature Rupture

*Gene Expression Regulation

Humans

Inflammation/*diagnosis

Mass Spectrometry

Neutrophils/metabolism

Obstetric Labor

Premature

Pregnancy

Pregnancy Complications

Premature Birth/*diagnosis/*metabolism

Protein Array Analysis/*methods

Proteins/chemistry

Proteome/chemistry

Proteomics/*methods

Spectrometry

Mass

Matrix-Assisted Laser Desorption-Ionization

Publikations- och innehållstyp

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