Butylated hydroxytoluene and N-acetylcysteine attenuates tumor necrosis factor-alpha (TNF-alpha) secretion and TNF-alpha mRNA expression in alveolar macrophages from human lung transplant recipients in vitro

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Mattsson Hultén, Lillemor,1951Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory (författare)

Butylated hydroxytoluene and N-acetylcysteine attenuates tumor necrosis factor-alpha (TNF-alpha) secretion and TNF-alpha mRNA expression in alveolar macrophages from human lung transplant recipients in vitro

Artikel/kapitelEngelska1998

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1998

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LIBRIS-ID:oai:gup.ub.gu.se/46920
https://gup.ub.gu.se/publication/46920URI

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Språk:engelska

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BACKGROUND: Tumor necrosis factor-alpha (TNF-alpha) is a polypeptide cytokine principally produced by macrophages/monocytes and commonly associated with inflammatory conditions. The present study was designed to investigate whether the antioxidants butylated hydroxytoluene (BHT) and N-acetylcysteine (NAC) modified TNF-alpha production in stimulated and unstimulated alveolar macrophages from lung transplant recipients in vitro. METHODS: The effects of BHT and NAC on TNF-alpha production were studied both with and without lipopolysaccharide (LPS) activation of alveolar macrophages from bronchoalveolar lavage fluid. TNF-alpha was quantitated in cell culture medium using an enzyme-linked immunosorbent assay. TNF-alpha mRNA expression was analyzed by quantitative reverse transcription-polymerase chain reaction on total RNA extracted from the incubated alveolar macrophages. RESULTS: In unstimulated alveolar macrophages, TNF-alpha levels were significantly reduced by incubation with BHT or NAC. When alveolar macrophages from patients with cytomegalovirus infection were incubated with BHT, TNF-alpha secretion was significantly lowered. A significant reduction of TNF-alpha levels in LPS-stimulated alveolar macrophages was obtained in the presence of BHT or NAC. Our data from quantitative reverse transcription-polymerase chain reaction showed that the observed decrease in protein levels of TNF-alpha was associated with a decrease in TNF-alpha mRNA expression. CONCLUSIONS: Our results indicate that antioxidant treatment may be an effective step to lower the inflammatory process caused by cytomegalovirus infection or in endotoxin (LPS)-activated macrophages. The therapeutic use of antioxidant compounds could, therefore, be of interest in conditions such as lung transplantation, in which oxidative stress and inflammation can contribute significantly to the loss of allograft function.

Ämnesord och genrebeteckningar

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Dermatologi och venereologi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Dermatology and Venereal Diseases hsv//eng
Acetylcysteine/*pharmacology
Adult
Antioxidants/*pharmacology
Butylated Hydroxytoluene/*pharmacology
Female
Gene Expression/drug effects
Graft Survival/drug effects/immunology
Heart Transplantation/*immunology
Heart-Lung Transplantation/*immunology
Humans
Lipopolysaccharides/immunology
Macrophage Activation/drug effects/immunology
Macrophages
Alveolar/*drug effects/immunology
Male
Middle Aged
RNA
Messenger/genetics
Tumor Necrosis Factor-alpha/genetics/*metabolism

Biuppslag (personer, institutioner, konferenser, titlar ...)

Lindmark, Helena,1969Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin,Institute of Internal Medicine (författare)
Scherstén, Henrik,1956Gothenburg University,Göteborgs universitet,Hjärt-kärlinstitutionen,Cardiovascular Institute (författare)
Wiklund, Olov,1943Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin,Institute of Internal Medicine(Swepub:gu)xwikol (författare)
Nilsson, Folke,1950Gothenburg University,Göteborgs universitet,Hjärt-kärlinstitutionen,Cardiovascular Institute(Swepub:gu)xnilfo (författare)
Riise, Gerdt C.,1956Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin,Institute of Internal Medicine(Swepub:gu)xriige (författare)
Göteborgs universitetWallenberglaboratoriet (creator_code:org_t)

Sammanhörande titlar

Ingår i:Transplantation66:3, s. 364-90041-1337

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https://gup.ub.gu.se/publication/46920

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Av författaren/redakt...: Mattsson Hultén, ...; Lindmark, Helena ...; Scherstén, Henri ...; Wiklund, Olov, 1 ...; Nilsson, Folke, ...; Riise, Gerdt C., ...

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin; och Dermatologi och ...

Artiklar i publikationen: Transplantation

Av lärosätet: Göteborgs universitet

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