Demonstration of P2Y4 purinergic receptors in the HT-29 human colon cancer cell line

• 1 The aim of the current study was to investigate the existence of P 2 Y(4) purinergic receptors in the HT-29 human colon cancer cell line. 2 We utilized Western blots and immunocytochemistry for the analysis. 3 Western blotting demonstrated two bands that could not be found after the antibody had been preabsorbed with the control peptide, suggesting that both bands are related to the P 2 Y(4) purinergic receptor. 4 Immunocytochemistry showed immunoreactivity for the P 2 Y(4) purinergic receptor localized in the cytoplasm of the HT-29 cells. 5 This is the first demonstration of the protein expression of P 2 Y(4) purinergic receptors in a human colon cancer cell line.

Nyckelord

Blotting

Western

Cytoplasm/metabolism

HT29 Cells/*metabolism

Humans

Immunohistochemistry

Receptors

Purinergic P2/*analysis

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