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A role of GM-CSF in...
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Laan, Martti,1971
(författare)
A role of GM-CSF in the accumulation of neutrophils in the airways caused by IL-17 and TNF-alpha
- Artikel/kapitelEngelska2003
Förlag, utgivningsår, omfång ...
Nummerbeteckningar
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LIBRIS-ID:oai:gup.ub.gu.se/50192
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https://gup.ub.gu.se/publication/50192URI
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Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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The T-cell cytokine interleukin (IL)-17 selectively accumulates neutrophils in murine airways in vivo and may thus constitute a link between activation of T-lymphocytes and accumulation of neutrophils. In this study, the authors evaluated the role of granulocyte macrophage-colony stimulating factor (GM-CSF) in accumulation of neutrophils in the airways caused by IL-17 and tumour necrosis factor (TNF)-alpha. In vitro, human (h) IL-17 concentration-dependently stimulated the release of GM-CSF protein (enzyme-linked immunosorbent assay) in human bronchial epithelial cells (16HBE). IL-17 also time-dependently stimulated the release of GM-CSF protein in venous endothelial (human umbilical vein endothelial cells) cells in vitro. Co-stimulation with IL-17 plus the pro-inflammatory cytokine TNF-alpha potentiated the release of GM-CSF protein in 16HBE cells. hIL-17 also enhanced the expression of GM-CSF messenger ribonucleic acid in 16HBE cells (reverse transcriptase polymerase chain reaction), with a similar order of magnitude as TNF-alpha. Conditioned cell medium from bronchial epithelial cells co-stimulated with hIL-17 plus TNF-alpha prolonged survival (trypan blue exclusion) of human neutrophils in vitro and this effect was blocked by an anti-GM-CSF antibody. In vivo, local co-stimulation with mouse IL-17 plus TNF-alpha caused an additive potentiation of the accumulation of neutrophils in bronchoalveolar lavage fluid from mouse airways and this effect was blocked by an anti-GM-CSF antibody given systemically. In conclusion, granulocyte macrophage-colony stimulating factor is involved in the accumulation of neutrophils in the airways caused by interleukin-17 and tumour necrosis factor-alpha, probably via effects on both recruitment and survival of neutrophils.
Ämnesord och genrebeteckningar
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MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin hsv//swe
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MEDICAL AND HEALTH SCIENCES Clinical Medicine hsv//eng
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Animals
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Bronchial Hyperreactivity/physiopathology
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Bronchoalveolar Lavage Fluid/cytology
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Cell Survival
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Cells
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Cultured
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Disease Models
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Animal
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Dose-Response Relationship
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Drug
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Granulocyte-Macrophage Colony-Stimulating Factor/analysis/*metabolism
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Humans
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Interleukin-7/*pharmacology
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Lymphocyte Activation/drug effects/physiology
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Male
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Mice
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Mice
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Inbred C57BL
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Neutrophil Activation/*drug effects
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Neutrophils/*drug effects/physiology
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Probability
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RNA
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Messenger/analysis/drug effects
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Sensitivity and Specificity
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Statistics
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Nonparametric
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Tumor Necrosis Factor-alpha/*pharmacology
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Prause, O.
(författare)
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Miyamoto, M.
(författare)
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Sjöstrand, Margareta,1947Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för lungmedicin och allergologi,Institute of Internal Medicine, Dept of Respiratory Medicine/Allergology(Swepub:gu)xsjosm
(författare)
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Hytonen, A. M.
(författare)
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Kaneko, T.
(författare)
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Lötvall, Jan,1956Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för lungmedicin och allergologi,Institute of Internal Medicine, Dept of Respiratory Medicine/Allergology(Swepub:gu)xlotja
(författare)
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Lindén, Anders,1961Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för lungmedicin och allergologi,Institute of Internal Medicine, Dept of Respiratory Medicine/Allergology(Swepub:gu)xlandd
(författare)
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Göteborgs universitetInstitutionen för invärtesmedicin, Avdelningen för lungmedicin och allergologi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:The European respiratory journal21:3, s. 387-930903-1936
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Laan, Martti, 19 ...
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Prause, O.
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Miyamoto, M.
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Sjöstrand, Marga ...
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Hytonen, A. M.
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Kaneko, T.
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Lötvall, Jan, 19 ...
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Lindén, Anders, ...
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