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A role of GM-CSF in...
A role of GM-CSF in the accumulation of neutrophils in the airways caused by IL-17 and TNF-alpha
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Laan, Martti, 1971 (författare)
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Prause, O. (författare)
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Miyamoto, M. (författare)
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- Sjöstrand, Margareta, 1947 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för lungmedicin och allergologi,Institute of Internal Medicine, Dept of Respiratory Medicine/Allergology
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Hytonen, A. M. (författare)
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Kaneko, T. (författare)
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- Lötvall, Jan, 1956 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för lungmedicin och allergologi,Institute of Internal Medicine, Dept of Respiratory Medicine/Allergology
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- Lindén, Anders, 1961 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för lungmedicin och allergologi,Institute of Internal Medicine, Dept of Respiratory Medicine/Allergology
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(creator_code:org_t)
- 2003
- 2003
- Engelska.
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Ingår i: The European respiratory journal. - 0903-1936. ; 21:3, s. 387-93
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- The T-cell cytokine interleukin (IL)-17 selectively accumulates neutrophils in murine airways in vivo and may thus constitute a link between activation of T-lymphocytes and accumulation of neutrophils. In this study, the authors evaluated the role of granulocyte macrophage-colony stimulating factor (GM-CSF) in accumulation of neutrophils in the airways caused by IL-17 and tumour necrosis factor (TNF)-alpha. In vitro, human (h) IL-17 concentration-dependently stimulated the release of GM-CSF protein (enzyme-linked immunosorbent assay) in human bronchial epithelial cells (16HBE). IL-17 also time-dependently stimulated the release of GM-CSF protein in venous endothelial (human umbilical vein endothelial cells) cells in vitro. Co-stimulation with IL-17 plus the pro-inflammatory cytokine TNF-alpha potentiated the release of GM-CSF protein in 16HBE cells. hIL-17 also enhanced the expression of GM-CSF messenger ribonucleic acid in 16HBE cells (reverse transcriptase polymerase chain reaction), with a similar order of magnitude as TNF-alpha. Conditioned cell medium from bronchial epithelial cells co-stimulated with hIL-17 plus TNF-alpha prolonged survival (trypan blue exclusion) of human neutrophils in vitro and this effect was blocked by an anti-GM-CSF antibody. In vivo, local co-stimulation with mouse IL-17 plus TNF-alpha caused an additive potentiation of the accumulation of neutrophils in bronchoalveolar lavage fluid from mouse airways and this effect was blocked by an anti-GM-CSF antibody given systemically. In conclusion, granulocyte macrophage-colony stimulating factor is involved in the accumulation of neutrophils in the airways caused by interleukin-17 and tumour necrosis factor-alpha, probably via effects on both recruitment and survival of neutrophils.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
Nyckelord
- Animals
- Bronchial Hyperreactivity/physiopathology
- Bronchoalveolar Lavage Fluid/cytology
- Cell Survival
- Cells
- Cultured
- Disease Models
- Animal
- Dose-Response Relationship
- Drug
- Granulocyte-Macrophage Colony-Stimulating Factor/analysis/*metabolism
- Humans
- Interleukin-7/*pharmacology
- Lymphocyte Activation/drug effects/physiology
- Male
- Mice
- Mice
- Inbred C57BL
- Neutrophil Activation/*drug effects
- Neutrophils/*drug effects/physiology
- Probability
- RNA
- Messenger/analysis/drug effects
- Sensitivity and Specificity
- Statistics
- Nonparametric
- Tumor Necrosis Factor-alpha/*pharmacology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Laan, Martti, 19 ...
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Prause, O.
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Miyamoto, M.
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Sjöstrand, Marga ...
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Hytonen, A. M.
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Kaneko, T.
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visa fler...
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Lötvall, Jan, 19 ...
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Lindén, Anders, ...
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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The European res ...
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Göteborgs universitet