Evidence of impaired adipogenesis in insulin resistance

Yang, Xiao Lin,1955Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för internmedicin,Institute of Internal Medicine, Dept of Medicine (författare)

To elucidate the roles of adipose tissue and skeletal muscle in the early development of insulin resistance, we characterized gene expression profiles of isolated adipose cells and skeletal muscle of non-diabetic insulin-resistant first-degree relatives of type 2 diabetic patients using oligonucleotide microarrays. About 600 genes and expressed sequence tags, which displayed a gene expression pattern of cell proliferation, were differentially expressed in the adipose cells. The differentially expressed genes in the skeletal muscle were mostly related to the cellular signal transduction and transcriptional regulation. To verify the microarray findings, we studied expression of genes participating in adipogenesis. The expression of Wnt signaling genes, WNT1, FZD1, DVL1, GSK3beta, beta-catenin, and TCF1, and adipogenic transcription factors, C/EBPalpha and beta and delta, PPARgamma, and SREBP-1, was reduced in the adipose tissue. The expression of adipose-specific proteins related to terminal differentiation, such as adiponectin and aP2, was reduced both in the adipose tissue and in the adipose cells isolated from portions of the biopsies. The adipose cells were enlarged in the insulin-resistant relatives and the cell size inversely correlated with the expression of the Wnt signaling genes, adiponectin, and aP2. Our findings suggest that insulin resistance is associated with an impaired adipogenesis.

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Endokrinologi och diabetes hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Endocrinology and Diabetes hsv//eng
Adipocytes/*metabolism/physiology
Adipose Tissue/cytology/*metabolism
Adult
Case-Control Studies
Cell Size
Cytoskeletal Proteins/biosynthesis
DNA-Binding Proteins/metabolism
Diabetes Mellitus
Type 2/genetics/metabolism
Family Health
Gene Expression Profiling
Glycogen Synthase Kinase 3/metabolism
Humans
Insulin Resistance/*physiology
Lymphoid Enhancer-Binding Factor 1
Male
Middle Aged
Muscle
Skeletal/metabolism/physiology
Oligonucleotide Array Sequence Analysis
Proto-Oncogene Proteins/genetics/metabolism
Regression Analysis
Signal Transduction
T Cell Transcription Factor 1
Trans-Activators/biosynthesis
Transcription Factors/metabolism
Wnt Proteins
Wnt1 Protein
*Zebrafish Proteins
beta Catenin

Jansson, Per-Anders,1961Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för internmedicin,Institute of Internal Medicine, Dept of Medicine(Swepub:gu)xjansp (författare)
Nagaev, Ivan,1962Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för internmedicin,Institute of Internal Medicine, Dept of Medicine(Swepub:gu)xnagiv (författare)
Jack, M. M. (författare)
Carvalho, Eugénia,1967Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för internmedicin,Institute of Internal Medicine, Dept of Medicine (författare)
Stibrant Sunnerhagen, Katharina,1957Gothenburg University,Göteborgs universitet,Institutionen för klinisk neurovetenskap,Institute of Clinical Neurosciences(Swepub:gu)xstika (författare)
Cam, M. C. (författare)
Cushman, S. W. (författare)
Smith, Ulf,1943Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för internmedicin,Institute of Internal Medicine, Dept of Medicine(Swepub:gu)xsmiul (författare)
Göteborgs universitetInstitutionen för invärtesmedicin, Avdelningen för internmedicin (creator_code:org_t)

Av författaren/redakt...: Yang, Xiao Lin, ...; Jansson, Per-And ...; Nagaev, Ivan, 19 ...; Jack, M. M.; Carvalho, Eugéni ...; Stibrant Sunnerh ...; visa fler...; Cam, M. C.; Cushman, S. W.; Smith, Ulf, 1943; visa färre...

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