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Derivation, charact...
Derivation, characterization, and differentiation of human embryonic stem cells.
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Heins, Nico (author)
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Englund, Mikael C. O., 1971 (author)
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Sjöblom, Cecilia (author)
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- Dahl, Ulf (author)
- Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk kemi/transfusionsmedicin,Institute of Laboratory Medicine, Dept of Clinical Chemistry/Transfusion Medicine
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- Tonning, Anna, 1978 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för infektionssjukdomar,Institute of Internal Medicine, Dept of Infectious Diseases
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- Bergh, Christina, 1953 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kvinnors och barns hälsa, Avdelningen för obstetrik och gynekologi,Institute for the Health of Women and Children, Dept of Obstetrics and Gynaecology
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- Lindahl, Anders, 1954 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk kemi/transfusionsmedicin,Institute of Laboratory Medicine, Dept of Clinical Chemistry/Transfusion Medicine
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- Hanson, Charles, 1958 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kvinnors och barns hälsa, Avdelningen för obstetrik och gynekologi,Institute for the Health of Women and Children, Dept of Obstetrics and Gynaecology
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- Semb, Henrik, 1959 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk kemi/transfusionsmedicin,Institute of Laboratory Medicine, Dept of Clinical Chemistry/Transfusion Medicine
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(creator_code:org_t)
- 2004
- 2004
- English.
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In: Stem cells (Dayton, Ohio). - 1066-5099. ; 22:3, s. 367-76
- Related links:
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https://gup.ub.gu.se...
Abstract
Subject headings
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- The derivation of human embryonic stem (hES) cells establishes a new avenue to approach many issues in human biology and medicine for the first time. To meet the increased demand for characterized hES cell lines, we present the derivation and characterization of six hES cell lines. In addition to the previously described immunosurgery procedure, we were able to propagate the inner cell mass and establish hES cell lines from pronase-treated and hatched blastocysts. The cell lines were extensively characterized by expression analysis of markers characteristic for undifferentiated and differentiated hES cells, karyotyping, telomerase activity measurement, and pluripotency assays in vitro and in vivo. Whereas three of the cell lines expressed all the characteristics of undifferentiated pluripotent hES cells, one cell line carried a chromosome 13 trisomy while maintaining an undifferentiated pluripotent state, and two cell lines, one of which carried a triploid karyotype, exhibited limited pluripotency in vivo. Furthermore, we clonally derived one cell line, which could be propagated in an undifferentiated pluripotent state.
Keyword
- Animals
- Blastocyst
- cytology
- metabolism
- Cell Differentiation
- physiology
- Cell Line
- Cell Lineage
- physiology
- Coculture Techniques
- Embryo
- cytology
- metabolism
- Fibroblasts
- cytology
- Humans
- Immunohistochemistry
- Karyotyping
- Mice
- Pluripotent Stem Cells
- cytology
- metabolism
- Stem Cell Transplantation
- Telomerase
- metabolism
- Transplantation
- Heterologous
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Heins, Nico
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Englund, Mikael ...
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Sjöblom, Cecilia
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Dahl, Ulf
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Tonning, Anna, 1 ...
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Bergh, Christina ...
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show more...
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Lindahl, Anders, ...
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Hanson, Charles, ...
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Semb, Henrik, 19 ...
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- Articles in the publication
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Stem cells (Dayt ...
- By the university
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University of Gothenburg