Monocyte viability on titanium and copper coated titanium.

• The role of apoptosis/cell death in the inflammatory response at the implanted materials is unexplored. Two surfaces with different cytotoxic potential and in vivo outcomes, titanium (Ti) and copper (Cu) were incubated in vitro with human monocytes and studied using a method to discriminate apoptotic and necrotic cells (Annexin V/PI staining). Further, staurosporine, a potent inducer of apoptosis, was added to the surface adherent monocytes. Lactate dehydrogenase (a marker of cell membrane injury) and TNF-alpha and IL-10, cytokines, previously suggested to play a major role in the monocyte apoptosis, were assayed in the culture medium. The results demonstrated that Ti surfaces displayed enhanced monocyte survival and production of IL-10 and TNF-alpha. Cu adherent cells exhibited apoptotic signs as early as 1h after incubation. In contrast to Ti, after 48 h the predominance of apoptotic cells switched to apoptotic/necrotic cells on Cu surfaces. Staurosporine treatment of Ti adherent cells mediated similar type of cell death. LDH and cytokine contents were low around Cu surfaces, partly explained by interference between Cu ions and LDH and cytokines. This study suggests that material properties rapidly influence the onset of human monocyte apoptosis and progression to late apoptosis/necrosis. Early detection of apoptosis and cell death may be important for the understanding of the biological response to implanted materials.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Medical Biotechnology -- Biomaterials Science (hsv//eng)

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine (hsv//eng)

Keyword

Annexin A5

pharmacology

Apoptosis

Biocompatible Materials

chemistry

Cell Adhesion

Cell Culture Techniques

methods

Cell Death

Cell Survival

Centrifugation

Coated Materials

Biocompatible

chemistry

Copper

chemistry

Cytokines

metabolism

Humans

Inflammation

Interleukin-10

metabolism

Kinetics

L-Lactate Dehydrogenase

metabolism

Monocytes

cytology

metabolism

Necrosis

Propidium

pharmacology

Staurosporine

pharmacology

Surface Properties

Time Factors

Titanium

chemistry

pharmacology

Tumor Necrosis Factor-alpha

metabolism

NATURAL SCIENCES

Publication and Content Type

ref (subject category)

art (subject category)