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  • Synnergren, Jane,1967Högskolan i Skövde,Institutionen för kommunikation och information (author)

Differentiating human embryonic stem cells express a unique housekeeping gene signature.

  • Article/chapterEnglish2007

Publisher, publication year, extent ...

  • 2007-02-01
  • Oxford University Press (OUP),2007

Numbers

  • LIBRIS-ID:oai:gup.ub.gu.se/50983
  • https://gup.ub.gu.se/publication/50983URI
  • https://doi.org/10.1634/stemcells.2006-0247DOI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-2004URI

Supplementary language notes

  • Language:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Housekeeping genes (HKGs) are involved in basic functions needed for the sustenance of the cell and are assumed to be constitutively expressed at a constant level. Based on these features, HKGs are frequently used for normalization of gene expression data. In the present study, we used the CodeLink Gene Expression Bioarray system to interrogate changes in gene expression occurring during differentiation of human ESCs (hESCs). Notably, in the three hESC lines used for the study, we observed that the RNA levels of 56 frequently used HKGs varied to a degree that rendered them inappropriate as reference genes. Therefore, we defined a novel set of HKGs specifically for hESCs. Here we present a comprehensive list of 292 genes that are stably expressed (coefficient of variation <20%) in differentiating hESCs. These genes were further grouped into high-, medium-, and low-expressed genes. The expression patterns of these novel HKGs show very little overlap with results obtained from somatic cells and tissues. We further explored the stability of this novel set of HKGs in independent, publicly available gene expression data from hESCs and observed substantial similarities with our results. Gene expression was confirmed by real-time quantitative polymerase chain reaction analysis. Taken together, these results suggest that differentiating hESCs have a unique HKG signature and underscore the necessity to validate the expression profiles of putative HKGs. In addition, this novel set of HKGs can preferentially be used as controls in gene expression analyses of differentiating hESCs.

Subject headings and genre

  • Animals
  • Biological Markers
  • metabolism
  • Cell Differentiation
  • genetics
  • Embryonic Stem Cells
  • cytology
  • metabolism
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Genes
  • Essential
  • Humans
  • Mice
  • RNA
  • Messenger
  • genetics
  • metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Gene expression

Added entries (persons, corporate bodies, meetings, titles ...)

  • Giesler, Theresa LGE Healthcare, Piscataway, NJ, United States (author)
  • Adak, SudeshnaGE John F. Welch Technology Centre Export Promotion Industrial Park, Bangalore, India (author)
  • Tandon, ReetiGE John F. Welch Technology Centre Export Promotion Industrial Park, Bangalore, India (author)
  • Noaksson, KarinCellartis AB, Göteborg, Sweden (author)
  • Lindahl, Anders,1954Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine,Department of Clinical Chemistry/Transfusion Medicine, Sahlgrenska University Hospital, Göteborg, Sweden(Swepub:gu)xlandy (author)
  • Nilsson, PatricHögskolan i Skövde,Institutionen för vård och natur(Swepub:his)nisp (author)
  • Nelson, DeirdreGE Global Research Center, Niskayuna, NY, United States (author)
  • Olsson, BjörnHögskolan i Skövde,Institutionen för kommunikation och information(Swepub:his)olsb (author)
  • Englund, Mikael C. O.,1971Cellartis AB, Göteborg, Sweden (author)
  • Sartipy, PeterCellartis AB, Göteborg, Sweden / Cellartis AB, Arvid Wallgrens Backe 20, SE-41346 Göteborg, Sweden(Swepub:his)sarp (author)
  • Abbot, StewartGE Global Research Center, Niskayuna, NY, United States (author)
  • Högskolan i SkövdeInstitutionen för kommunikation och information (creator_code:org_t)

Related titles

  • In:Stem cells (Dayton, Ohio): Oxford University Press (OUP)25:2, s. 473-801066-50991549-4918

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