LEPREL1, a novel ER and Golgi resident member of the Leprecan family.

• We have identified a novel protein, Leprecan-like 1 (LEPREL1), with profound similarity to the Leprecan family of proteoglycans. The genomic organization of the Leprecan gene family was found to be highly conserved. Expression analysis shows that LEPREL1 is expressed in most tissues as a 3.4 kb transcript encoding an 80 kDa protein. A LEPREL1 specific antibody stains many cell types including adipocytes and neuroendocrine cells of the gastrointestinal epithelium. Muscle tissue contains a specific 6.5 kb transcript and a 200 kDa protein. The 3.4 kb LEPREL1 transcript encodes a 708 amino acid protein containing a signal sequence, four tetratricopeptide repeats (TPRs), a leucine zipper, a P-loop, a prolyl 4-hydroxylase alpha domain (P4Halpha), and a C-terminal KDEL ER-retention motif. LEPREL1 is localized to the ER and Golgi network and over-expressing it affects normal protein disulfide isomerase staining patterns in the ER.

Ämnesord

NATURVETENSKAP  -- Biologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

Nyckelord

Amino Acid Sequence

Animals

Cell Line

Tumor

Endoplasmic Reticulum

metabolism

ultrastructure

Fibrosarcoma

genetics

metabolism

Golgi Apparatus

metabolism

ultrastructure

Humans

Membrane Glycoproteins

chemistry

genetics

metabolism

Mice

Molecular Sequence Data

Organ Specificity

Proteoglycans

chemistry

genetics

metabolism

Sequence Analysis

Protein

Sequence Homology

Amino Acid

Species Specificity

Tissue Distribution

Hydroxylase

TPR

Author Keywords: Protein disulfide isomerase

P4Hα

Liposarcoma

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