Translocated in liposarcoma (TLS) is a substrate for fibroblast growth factor receptor-1.

• Binding of fibroblast growth factor (FGF) to the high affinity receptor-1 (FGFR-1) leads to activation of its endogenous tyrosine kinase activity. A number of substrates for the FGFR-1 kinase have been identified. Among those, FGF receptor-substrate-2 (FRS-2) was identified by virtue of its interaction with p13suc, a yeast protein involved in cell cycle regulation. We have used immobilized p13suc to identify a new substrate for FGRF-1, which is identical to "translocated in liposarcoma" (TLS). TLS is a RNA/DNA-binding protein which occurs in fusion products with different transcription factors in a variety of solid tumours. We show that TLS is tyrosine phosphorylated in intact cells by a number of different growth factors, indicating a role in growth regulation.

Keyword

Adaptor Proteins

Signal Transducing

Animals

Cell Fractionation

Fibroblast Growth Factors

metabolism

Liposarcoma

metabolism

Membrane Proteins

metabolism

Mice

Phosphoproteins

metabolism

Phosphorylation

Receptor Protein-Tyrosine Kinases

metabolism

Receptor

Fibroblast Growth Factor

Type 1

Receptors

Fibroblast Growth Factor

metabolism

Swiss 3T3 Cells

Tyrosine

metabolism

Animals

Publication and Content Type

ref (subject category)

art (subject category)