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Gelatinases and their tissue inhibitors during human ovulation: increased expression of tissue inhibitor of matrix metalloproteinase-1

Lind, Anna Karin, 1962 (författare)
Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences
Dahm-Kähler, Pernilla, 1964 (författare)
Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences
Weijdegard, B (författare)
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Sundfeldt, Karin, 1962 (författare)
Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences
Brännström, Mats, 1958 (författare)
Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences
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 (creator_code:org_t)
2006
2006
Engelska.
Ingår i: Mol Hum Reprod. ; 12:12, s. 725-36
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
Stäng  
  • Remodelling of the extracellular matrix (ECM) of the follicular wall by matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs) has been suggested to be crucial in ovulation. To investigate the expression of the gelatinases, MMP-2 and MMP-9, together with their inhibitors, TIMP-2 and TIMP-1, in the perifollicular ovarian stroma from women just before and during ovulation, we obtained biopsies of the stroma adjacent to the leading follicle. Laparoscopic surgery was performed either before the LH peak or at any of three intervals after ovulation triggering by hCG. Immunoblotting, immunohistochemistry and quantitative RT-PCR were performed. All four proteins were expressed by immunoblots, with no detectable changes in the expression of MMP-2, MMP-9 and TIMP-2. Scattered immunostaining for MMP-9 and TIMP-2 was seen, and MMP-2 was demonstrated in a concentric layer. A significant increase in TIMP-1 protein and mRNA was seen during the three ovulatory phases, and a strong and patchy immunostaining for TIMP-1 was shown. This is the first study that has demonstrated an ovulation-associated expression of these ECM-remodelling enzymes around the human follicle at ovulation. The increased expression of TIMP-1 may reflect a specific temporal inhibition of collagenolysis and thereby a time-dependent regulation of ECM breakdown in areas surrounding the apex of the follicle.

Nyckelord

Adult
Blotting
Western
Chorionic Gonadotropin/pharmacology
Enzyme Induction
Extracellular Matrix/metabolism
Female
Granulosa Cells/metabolism
Humans
Immunoenzyme Techniques
Matrix Metalloproteinase 2/*physiology
Matrix Metalloproteinase 9/*physiology
Ovarian Follicle/metabolism
Ovulation/*physiology
Ovulation Induction
RNA
Messenger/biosynthesis
Reverse Transcriptase Polymerase Chain Reaction
Stromal Cells/metabolism
Theca Cells/metabolism
Tissue Inhibitor of Metalloproteinase-1/biosynthesis/genetics/*physiology
Tissue Inhibitor of Metalloproteinase-2/physiology

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