Neural cell adhesion molecule-deficient beta-cell tumorigenesis results in diminished extracellular matrix molecule expression and tumour cell-matrix adhesion

Håkansson, Joakim,1975Gothenburg University,Göteborgs universitet,Institutionen för medicinsk och fysiologisk kemi,Institute of Medical Biochemistry,University of Gothenburg (författare)

To understand by which mechanism neural cell adhesion molecule (N-CAM) limits beta tumour cell disaggregation and dissemination, we searched for potential downstream genes of N-CAM during beta tumour cell progression by gene expression profiling. Here, we show that N-CAM-deficient beta-cell tumorigenesis is associated with changes in the expression of genes involved in cell-matrix adhesion and cytoskeletal dynamics, biological processes known to affect the invasive and metastatic behaviour of tumour cells. The extracellular matrix (ECM) molecules emerged as the primary target, i.e. N-CAM deficiency resulted in down-regulated mRNA expression of a broad range of ECM molecules. Consistent with this result, deficient deposition of major ECM stromal components, such as fibronectin, laminin 1 and collagen IV, was observed. Moreover, N-CAM-deficient tumour cells displayed defective matrix adhesion. These results offer a potential mechanism for tumour cell disaggregation during N-CAM-deficient beta tumour cell progression. Prospective consequences of these findings for the role of N-CAM in beta tumour cell dissemination are discussed.

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
Animals
Cell Transformation
Neoplastic
Disease Progression
Extracellular Matrix Proteins
metabolism
Gene Expression Profiling
Insulinoma
metabolism
pathology
Mice
Mice
Inbred C57BL
Mice
Knockout
Neural Cell Adhesion Molecules
genetics
physiology
Oligonucleotide Array Sequence Analysis
RNA
Messenger
genetics
metabolism
Reverse Transcriptase Polymerase Chain Reaction
Tumor Markers
Biological
genetics
metabolism
metabolism

Xian, Xiaojie,1971Gothenburg University,Göteborgs universitet,Institutionen för medicinsk och fysiologisk kemi,Institute of Medical Biochemistry,University of Gothenburg(Swepub:gu)xxiaxi (författare)
He, LiqunGothenburg University,Göteborgs universitet,Institutionen för medicinsk och fysiologisk kemi,Institute of Medical Biochemistry,University of Gothenburg (författare)
Ståhlberg, Anders,1975Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)xsandw (författare)
Nelander, Sven,1974Gothenburg University,Göteborgs universitet,Institutionen för medicinsk och fysiologisk kemi,Institute of Medical Biochemistry,University of Gothenburg(Swepub:gu)xnelsv (författare)
Samuelsson, Tore,1951Gothenburg University,Göteborgs universitet,Institutionen för medicinsk och fysiologisk kemi,Institute of Medical Biochemistry,University of Gothenburg(Swepub:gu)xsamto (författare)
Kubista, Mikael,1961Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)mbtmk (författare)
Semb, Henrik,1959Gothenburg University,Göteborgs universitet,Institutionen för medicinsk och fysiologisk kemi,Institute of Medical Biochemistry,University of Gothenburg (författare)
Göteborgs universitetInstitutionen för medicinsk och fysiologisk kemi (creator_code:org_t)

Ingår i:Tumour Biology: Springer Science and Business Media LLC26:2, s. 103-1121010-42831423-0380

Av författaren/redakt...: Håkansson, Joaki ...; Xian, Xiaojie, 1 ...; He, Liqun; Ståhlberg, Ander ...; Nelander, Sven, ...; Samuelsson, Tore ...; visa fler...; Kubista, Mikael, ...; Semb, Henrik, 19 ...; visa färre...

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