Quantitative proteomics analysis of the secretory pathway.

• We report more than 1400 proteins of the secretory-pathway proteome and provide spatial information on the relative presence of each protein in the rough and smooth ER Golgi cisternae and Golgi-derived COPI vesicles. The data support a role for COPI vesicles in recycling and cisternal maturation, showing that Golgi-resident proteins are present at a higher concentration than secretory cargo. Of the 1400 proteins, 345 were identified as previously uncharacterized. Of these, 230 had their subcellular location deduced by proteomics. This study provides a comprehensive catalog of the ER and Golgi proteomes with insight into their identity and function.

Nyckelord

Animals

Coat Protein Complex I

Endoplasmic Reticulum

chemistry

Golgi Apparatus

chemistry

Liver

chemistry

cytology

Protein Transport

Proteins

analysis

isolation & purification

Proteomics

Rats

SNARE Proteins

isolation & purification

Tandem Mass Spectrometry

rab GTP-Binding Proteins

genetics

isolation & purification

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)