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  • Wettergren, Yvonne,1957 (författare)

Low expression of reduced folate carrier-1 and folylpolyglutamate synthase correlates with lack of a deleted in colorectal carcinoma mRNA splice variant in normal-appearing mucosa of colorectal carcinoma patients

  • Artikel/kapitelEngelska2005

Förlag, utgivningsår, omfång ...

  • Elsevier BV,2005

Nummerbeteckningar

  • LIBRIS-ID:oai:gup.ub.gu.se/52529
  • https://gup.ub.gu.se/publication/52529URI
  • https://doi.org/10.1016/j.cdp.2005.06.006DOI
  • https://research.chalmers.se/publication/52529URI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-80631URI

Kompletterande språkuppgifter

  • Språk:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • BACKGROUND: Cellular folate deficiency leads to DNA strand breaks, mutations, and aberrant methylation and might be a risk factor for colorectal cancer (CRC). The putative tumor suppressor gene deleted in colorectal carcinoma (DCC) is one of several genes the expression of which seems to be affected by the folate concentration at the tissue level. Decreased expression of DCC may be caused by LOH or hypermethylation, i.e. by events that might be linked to folate deficiency. The purpose of this study was to analyze if the folate level and the gene expression levels of reduced folate carrier (RFC-1) and folylpolyglutamate synthase (FPGS) had impact on the expression of DCC splice variants. METHODS: Quantification of RFC-1 and FPGS expression in mucosa of 53 CRC patients was performed using real-time PCR whereas DCC splicing variants were detected by automated capillary gel electrophoresis. Total reduced folate concentration was measured with the FdUMP-binding assay (n = 22). RESULTS: Significantly higher expression levels of RFC-1 (p = 0.026) and FPGS (p = 0.05) were found in mucosa expressing the splice variant DCC342 compared to mucosa that did not. Furthermore, multivariate analysis showed that RFC-1 and FPGS (r = 0.49, p = 0.01) as well as folate and RFC-1 (r = 0.56, p = 0.023) were correlated only in mucosa expressing DCC342. CONCLUSIONS: In conclusion, the present study points to a potential influence of folates in regulating DCC expression at multiple levels involving post-transcriptional pathways. The results may provide a basis for a detailed investigation of molecular mechanisms involved in folate regulation of DCC expression.

Ämnesord och genrebeteckningar

  • MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Kirurgi hsv//swe
  • MEDICAL AND HEALTH SCIENCES Clinical Medicine Surgery hsv//eng
  • Adenocarcinoma/chemistry/*genetics/pathology
  • Adult
  • Aged
  • Aged
  • 80 and over
  • *Alternative Splicing
  • Amino Acid Sequence
  • Colorectal Neoplasms/chemistry/*genetics/pathology
  • Female
  • Folic Acid Deficiency/genetics
  • Gene Expression Regulation
  • Neoplastic
  • Genes
  • DCC/genetics
  • Humans
  • Intestinal Mucosa/*chemistry
  • Male
  • Membrane Transport Proteins/analysis/*genetics
  • Middle Aged
  • Molecular Sequence Data
  • Multivariate Analysis
  • Peptide Synthases/analysis/*genetics
  • RNA
  • Neoplasm/*genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sweden
  • Neoplasm/*genetics

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Odin, Elisabeth,1955Gothenburg University,Göteborgs universitet,Institutionen för de kirurgiska disciplinerna, Avdelningen för kirurgi,Institute of Surgical Sciences, Department of Surgery,University of Gothenburg(Swepub:gu)xodiel (författare)
  • Nilsson, Staffan,1956Gothenburg University,Göteborgs universitet,Institutionen för matematiska vetenskaper, matematisk statistik,Department of Mathematical Sciences, Mathematical Statistics,University of Gothenburg,Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)staffan (författare)
  • Willen, Roger,1939Uppsala universitet,Institutionen för genetik och patologi (författare)
  • Carlsson, Göran,1951 (författare)
  • Gustavsson, Bengt,1947Gothenburg University,Göteborgs universitet,Institutionen för de kirurgiska disciplinerna, Avdelningen för kirurgi,Institute of Surgical Sciences, Department of Surgery,University of Gothenburg(Swepub:gu)xguben (författare)
  • Göteborgs universitetInstitutionen för de kirurgiska disciplinerna, Avdelningen för kirurgi (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Cancer Detect Prev: Elsevier BV29:4, s. 348-550361-090X1525-1500

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