Procedure-induced inflammation and endothelial cell activation in an artificially ventilated and circulated porcine double-lung model

• Systemic inflammation is induced during extracorporeal circulation, resulting in an increased bleeding tendency and endothelial cell activation. Lungs from seven piglets were perfused by autologous blood in an extracorporeal circuit, where the lungs and the left atrium were attached to polyvinyl chloride (PVC) tubings and the blood circulated by a roller pump. The trachea was intubated and attached to a ventilator. The lungs maintained good gas exchange, despite a slight increase in lactate levels. Plasma tPA increased slightly over time, suggesting endothelial cell activation. Activation of inflammatory systems was reflected in increased levels of plasma interleukin (IL)-6 and IL-10. A model for the study of lung endothelial activation during extracorporeal circulation has been shown to be reproducible. The lung tissue was shown to be capable of gas exchange and activation of endothelial cells and procedure-induced inflammation were noted.

Nyckelord

Animals

Endothelium

Vascular/cytology/metabolism

Enzyme-Linked Immunosorbent Assay

Equipment Design

Extracorporeal Circulation/*adverse effects

Female

Inflammation/prevention & control

Interleukin-10/blood

Interleukin-6/blood

Intubation

Intratracheal

Lung/*physiology

Models

Animal

Pulmonary Gas Exchange

Respiration

Artificial/*methods

Statistics

Nonparametric

Swine

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)