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Vaccine-induced imm...
Vaccine-induced immunity against Helicobacter pylori infection is impaired in IL-18-deficient mice.
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- Akhiani, Aliasghar, 1957 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk immunologi,Institute of Laboratory Medicine, Dept of Clinical Immunology
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- Schön, Karin, 1962 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk immunologi,Institute of Laboratory Medicine, Dept of Clinical Immunology
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- Lycke, Nils Y, 1954 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk immunologi,Institute of Laboratory Medicine, Dept of Clinical Immunology
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(creator_code:org_t)
- 2004
- 2004
- Engelska.
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Ingår i: Journal of immunology. - 0022-1767. ; 173:5, s. 3348-56
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- Protective immunity against Helicobacter pylori infection in mice has been associated with a strong Th1 response, involving IL-12 as well as IFN-gamma, but recent studies have also demonstrated prominent eosinophilic infiltration, possibly linked to local Th2 activity in the gastric mucosa. In this study we investigated the role of IL-18, because this cytokine has been found to be a coregulator of Th1 development as well as involved in Th2-type responses with local eotaxin production that could influence gastric eosinophilia and resistance to infection. We found that IL-18(-/-) mice failed to develop protection after oral immunization with H. pylori lysate and cholera toxin adjuvant, indicating an important role of IL-18 in protection. Well-protected C57BL/6 wild-type (WT) mice demonstrated substantial influx of CD4(+) T cells and eosinophilic cells in the gastric mucosa, whereas IL-18(-/-) mice had less gastritis, few CD4(+) T cells, and significantly reduced numbers of eosinophilic cells. T cells in well-protected WT mice produced increased levels of IFN-gamma and IL-18 to recall Ag. By contrast, unprotected IL-18(-/-) mice exhibited significantly reduced gastric IFN-gamma and specific IgG2a Ab levels. Despite differences in gastric eosinophilic cell infiltration, protected WT and unprotected IL-18(-/-) mice had comparable levels of local eotaxin, suggesting that IL-18 influences protection via Th1 development and IFN-gamma production rather than through promoting local production of eotaxin and eosinophilic cell infiltration.
Nyckelord
- Animals
- Antigens
- Bacterial
- immunology
- Chemokines
- CC
- metabolism
- Gastritis
- immunology
- metabolism
- Helicobacter Infections
- immunology
- prevention & control
- Helicobacter pylori
- immunology
- Interferon Type II
- metabolism
- Interleukin-18
- deficiency
- genetics
- immunology
- metabolism
- Mice
- Vaccines
- immunology
Publikations- och innehållstyp
- ref (ämneskategori)
- for (ämneskategori)
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