Low density and high affinity of platelet [3H]paroxetine binding in women with bulimia nervosa.

Ekman, Agneta,1961Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för farmakologi,Institute of Neuroscience and Physiology, Department of Pharmacology (author)

Impaired serotonin transmission has been suggested to be implicated in the pathophysiology of bulimia nervosa. As an indirect measure of brain serotonergic activity, the binding of tritiated ligands to platelet serotonin transporters has been studied in bulimia nervosa as well as in other putatively serotonin-related psychiatric disorders. In this study, the density and affinity of platelet serotonin transporters were assessed in 20 women meeting the DSM-IV criteria for bulimia nervosa and in 14 controls without previous or ongoing eating disorder using [(3)H]paroxetine as a ligand. In comparison to controls, women with bulimia nervosa had a significantly reduced number of platelet binding sites (B(max) = 721 +/- 313 vs. 1145 +/- 293 fmol/mg protein) and an increase in the affinity for the ligand demonstrated by a lower dissociaton constant (K(d) = 33 +/- 10 vs. 44 +/- 10 pM). A significant correlation between B(max) and K(d) values was found in patients but not in controls. Our results support the notion that bulimia nervosa is associated with a reduction in platelet serotonin transporter density. In addition, our study is the first to report that this reduced transporter density in women with bulimia nervosa is accompanied by an increase in the affinity of the transporter for the ligand.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Fysiologi hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Physiology hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Psykiatri hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Psychiatry hsv//eng
Adult
Blood Platelets
metabolism
Brain
physiopathology
Bulimia
diagnosis
physiopathology
psychology
Female
Humans
Paroxetine
pharmacokinetics
Radioligand Assay
Reference Values
Serotonin
physiology
Serotonin Plasma Membrane Transport Proteins
physiology
Tritium
women
[H-3]paroxetine binding
bulimia nervosa
platelets
serotonin transporter
K-d
B-max

Sundblad-Elverfors, Charlotta,1959Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för farmakologi,Institute of Neuroscience and Physiology, Department of Pharmacology (author)
Landén, Mikael,1966Karolinska Institutet(Swepub:gu)xlandt (author)
Eriksson, TomasLund University,Lunds universitet,Forskningsgruppen för biologisk psykiatri,Forskargrupper vid Lunds universitet,The Biological Psychiatry Research Group,Lund University Research Groups(Swepub:lu)psyk-ter (author)
Eriksson, Elias,1956Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för farmakologi,Institute of Neuroscience and Physiology, Department of Pharmacology(Swepub:gu)xereli (author)
Göteborgs universitetInstitutionen för neurovetenskap och fysiologi, sektionen för farmakologi (creator_code:org_t)

By the author/editor: Ekman, Agneta, 1 ...; Sundblad-Elverfo ...; Landén, Mikael, ...; Eriksson, Tomas; Eriksson, Elias, ...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Physiology

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Psychiatry

By the university: University of Gothenburg; Lund University; Karolinska Institutet

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