Effect of acidosis on expression of mesothelial cell plasminogen activator inhibitor type-1.

• BACKGROUND: Abdominal mesothelial cells are exposed to carbon dioxide during laparoscopy. Previous data indicate that carbon dioxide increases release and expression of plasminogen activator inhibitor type-1 (PAI-1) and induces acidification. METHODS: To assess the impact resulting from a range of pH, human mesothelial cells were exposed to culturing media balanced to pH levels of 6.0 to 8.0 for 90 min. Samples from cell media were withdrawn at several time points. Concentrations of PAI-1 and PAI-1 activity were measured using enzyme-linked immunoassay techniques. To focus on the effect of clinically relevant pH, cells were subjected to pH 6.4 and 7.4. Samples were withdrawn for PAI-1 assessments and for PAI-1 mRNA analyses. RESULTS: During exposure to various levels of pH, PAI-1 secretion and activity were variable. However, 5 h after exposure, greater concentration and activity of PAI-1 were observed in acidified cultures. More PAI-1 mRNA was isolated after exposure of cells to a pH of 6.4, apparently indicating transcriptional regulation. CONCLUSIONS: Mesothelial cells seem to respond to acidification by an increased release and production of PAI-1 in vitro.

Nyckelord

Acidosis

metabolism

pathology

Carbon Dioxide

pharmacology

Cells

Cultured

Enzyme-Linked Immunosorbent Assay

Epithelial Cells

drug effects

metabolism

Humans

Hydrogen-Ion Concentration

Peritoneum

drug effects

metabolism

pathology

Plasminogen Activator Inhibitor 1

genetics

metabolism

RNA

Messenger

metabolism

Time Factors

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