The nonerythropoietic asialoerythropoietin protects against neonatal hypoxia-ischemia as potently as erythropoietin.

Wang, Xiaoyang,1965Gothenburg University,Göteborgs universitet,Institutionen för fysiologi och farmakologi, Avdelningen för fysiologi,Institute of Physiology and Pharmacology, Dept of Physiology (author)

Recently, erythropoietin (EPO) and the nonerythropoietic derivative asialoEPO have been linked to tissue protection in the nervous system. In this study, we tested their effects in a model of neonatal hypoxia-ischemia (HI) in 7-day-old rats (unilateral carotid ligation and exposure to 7.7% O(2) for 50 min). EPO (10 U/g body weight = 80 ng/g; n = 24), asialoEPO (80 ng/g; n = 23) or vehicle (phosphate-buffered saline with 0.1% human serum albumin; n = 24) was injected intraperitoneally 4 h before HI. Both drugs were protective, as judged by measuring the infarct volumes, neuropathological score and gross morphological score. The infarct volumes were significantly reduced by both EPO (52%) and asialoEPO (55%) treatment, even though the plasma levels of asialoEPO had dropped below the detection limit (1 pm) at the onset of HI, while those of EPO were in the nanomolar range. Thus, a brief trigger by asialoEPO before the insult appears to be sufficient for protection. Proteomics analysis after asialoEPO treatment alone (no HI) revealed at least one differentially up-regulated protein, synaptosome-associated protein of 25 kDa (SNAP-25). Activation (phosphorylation) of ERK was significantly reduced in asialoEPO-treated animals after HI. EPO and the nonerythropoietic asialoEPO both provided significant and equal neuroprotection when administered 4 h prior to HI in 7-day-old rats. The protection might be related to reduced ERK activation and up-regulation of SNAP-25.

Zhu, Changlian,1964Gothenburg University,Göteborgs universitet,Institutionen för fysiologi och farmakologi, Avdelningen för fysiologi,Institute of Physiology and Pharmacology, Dept of Physiology(Swepub:gu)xzhuch (author)
Wang, XinhuaGothenburg University,Göteborgs universitet,Institutionen för fysiologi och farmakologi, Avdelningen för fysiologi,Institute of Physiology and Pharmacology, Dept of Physiology (author)
Gammeltoft Gerwien, Jens (author)
Schrattenholz, Andre (author)
Sandberg, Mats,1953Gothenburg University,Göteborgs universitet,Institutionen för fysiologi och farmakologi, Avdelningen för medicinsk fysik,Institute of Physiology and Pharmacology, Dept of Medical Biophysics(Swepub:gu)xsamat (author)
Leist, Marcel (author)
Blomgren, Klas,1963Gothenburg University,Göteborgs universitet,Institutionen för kvinnors och barns hälsa, Avdelningen för pediatrik,Institutionen för fysiologi och farmakologi, Avdelningen för fysiologi,Institute for the Health of Women and Children, Dept of Paediatrics,Institute of Physiology and Pharmacology, Dept of Physiology(Swepub:gu)xblokl (author)
Göteborgs universitetInstitutionen för fysiologi och farmakologi, Avdelningen för fysiologi (creator_code:org_t)

By the author/editor: Wang, Xiaoyang, ...; Zhu, Changlian, ...; Wang, Xinhua; Gammeltoft Gerwi ...; Schrattenholz, A ...; Sandberg, Mats, ...; show more...; Leist, Marcel; Blomgren, Klas, ...; show less...

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.