WFRF:(Henry Stephen)
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- Ångström, Jonas,1950Gothenburg University,Göteborgs universitet,Institutionen för medicinsk och fysiologisk kemi,Institute of Medical Biochemistry (författare)
Default biosynthesis pathway for blood group-related glycolipids in human small intestine as defined by structural identification of linear and branched glycosylceramides in a group O Le(a-b-) nonsecretor.
- Artikel/kapitelEngelska2004
Förlag, utgivningsår, omfång ...
- 2003-09-26
- Oxford University Press (OUP),2004
Nummerbeteckningar
- LIBRIS-ID:oai:gup.ub.gu.se/61512
- https://gup.ub.gu.se/publication/61512URI
- https://doi.org/10.1093/glycob/cwh003DOI
Kompletterande språkuppgifter
- Språk:engelska
Ingår i deldatabas
- SwePubSwePub
Klassifikation
Anmärkningar
- Glycoconjugates of the GI tract are important for microbial interactions. The expression of histo-blood group glycosyltransferases governs both the expression of blood group determinants and in part the structure and size of the glycoconjugates. Using neutral glycolipids isolated from the small intestine of a rare blood group O Le(a-b-) ABH secretor-negative (nonsecretor) individual we were able to map the "default" pathway of the individual lacking ABO, Lewis, and secretor glycosyltransferases. Structures were deduced with combined analysis of mass spectrometry (MALDI-TOF and ESI-MS/MS), and 1H NMR (500 and 600 MHz). All structures present at a level >5% were structurally resolved and included two extended structures: Galbeta4(Fucalpha3)GlcNAcbeta3(Galbeta4[Fucalpha3]GlcNAcbeta6)Galbeta4GlcNAcbeta3Galbeta4Glcbeta1Cer and Galbeta3GlcNAcbeta3(Galbeta4[Fucalpha3]GlcNAcbeta6)Galbeta3GlcNAcbeta3Galbeta4Glcbeta1Cer. The first, a novel component, is based on a type 2 chain and bears the Lex glycotopes on both its branches. The second, a major component, is based on a type 1 chain, which bears a 3-linked type 1 precursor (Lec) glycotope and a 6-linked Lex glycotope on its branches. This latter structure is identical to that previously isolated from plasma and characterized by MS and GC-MS but not by NMR. Structural resolution of these structures was supported by reanalysis of the blood group H-active decaosylceramides previously isolated from rat small intestine. Other minor linear monofucosylated penta-, hepta-, and difucosylated octaosylceramides, some bearing blood group determinants, were also identified. The cumulative data were used to define a default biosynthesis pathway where it can be seen that carbohydrate chain extension, in the absence of blood group glycosyltransferases, is controlled and regulated by non-blood group fucosylation and branching with type 2 Galbeta4GlcNAc branches.
Ämnesord och genrebeteckningar
- Animals
- Carbohydrate Conformation
- Carbohydrate Sequence
- Glycolipids
- biosynthesis
- Humans
- Intestine
- Small
- metabolism
- Lewis Blood-Group System
- chemistry
- Magnetic Resonance Spectroscopy
- methods
- Molecular Sequence Data
- Oligosaccharides
- biosynthesis
- chemistry
- Rats
- Spectrometry
- Mass
- Electrospray Ionization
- methods
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Larsson, Thomas,1947Gothenburg University,Göteborgs universitet,Institutionen för medicinsk och fysiologisk kemi,Institute of Medical Biochemistry(Swepub:gu)xlarth (författare)
- Hansson, Gunnar C.,1951Gothenburg University,Göteborgs universitet,Institutionen för medicinsk och fysiologisk kemi,Institute of Medical Biochemistry(Swepub:gu)xhagun (författare)
- Karlsson, Karl-Anders,1935Gothenburg University,Göteborgs universitet,Institutionen för medicinsk och fysiologisk kemi,Institute of Medical Biochemistry(Swepub:gu)xkarlk (författare)
- Henry, Stephen (författare)
- Göteborgs universitetInstitutionen för medicinsk och fysiologisk kemi (creator_code:org_t)
Sammanhörande titlar
- Ingår i:Glycobiology: Oxford University Press (OUP)14:1, s. 1-120959-66581460-2423
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- Glycobiology
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- Göteborgs universitet
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