Microarray analysis of blood microvessels from PDGF-B and PDGF-Rbeta mutant mice identifies novel markers for brain pericytes.

Bondjers, Cecilia,1974Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology (författare)

Normal blood microvessels are lined by pericytes, which contribute to microvessel development and stability through mechanisms that are poorly understood. Pericyte deficiency has been implicated in the pathogenesis of microvascular abnormalities associated with diabetes and tumors. However, the unambiguous identification of pericytes is still a problem because of cellular heterogeneity and few available molecular markers. Here we describe an approach to identify pericyte markers based on transcription profiling of pericyte-deficient brain microvessels isolated from platelet-derived growth factor (PDGF-B)-/- and PDGF beta receptor (PDGFRbeta)-/- mouse mutants. The approach was validated by the identification of known pericyte markers among the most down-regulated genes in PDGF-B-/- and PDGFRbeta-/- microvessels. Of candidates for novel pericyte markers, we selected ATP-sensitive potassium-channel Kir6.1 (also known as Kcnj8) and sulfonylurea receptor 2, (SUR2, also known as Abcc9), both part of the same channel complex, as well as delta homologue 1 (DLK1) for in situ hybridization, which demonstrated their specific expression in brain pericytes of mouse embryos. We also show that Kir6.1 is highly expressed in pericytes in brain but undetectable in pericytes in skin and heart. The three new brain pericyte markers are signaling molecules implicated in ion transport and intercellular signaling, potentially opening new windows on pericyte function in brain microvessels.

He, Liqun (författare)
Takemoto, Minoru (författare)
Norlin, Jenny (författare)
Asker, Noomi,1968Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology(Swepub:gu)xaskno (författare)
Hellström, Mats (författare)
Lindahl, Per,1967Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Wallenberg Laboratory,Institute of Medicine, Department of Molecular and Clinical Medicine,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology(Swepub:gu)xlindp (författare)
Betsholtz, Christer,1959Karolinska Institutet (författare)
Göteborgs universitetInstitutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi (creator_code:org_t)

Ingår i:The FASEB journal : official publication of the Federation of American Societies for Experimental Biology: Wiley20:10, s. 1703-51530-6860
Ingår i:The FASEB Journal: Wiley20:10, s. 1703-50892-6638

Av författaren/redakt...: Bondjers, Cecili ...; He, Liqun; Takemoto, Minoru; Norlin, Jenny; Asker, Noomi, 19 ...; Hellström, Mats; visa fler...; Lindahl, Per, 19 ...; Betsholtz, Chris ...; visa färre...

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