Sökning: WFRF:(Chávez de Paz Luis 1974) >
Response to alkalin...
Response to alkaline stress by root canal bacteria in biofilms. : Viability and mechanisms of response to alkaline stress by selected root canal bacteria.
-
Chávez de Paz, Luis, 1974 (författare)
-
- Bergenholtz, Gunnar, 1939 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för odontologi,Institute of Odontology
-
- Dahlén, Gunnar, 1944 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för odontologi,Institute of Odontology
-
visa fler...
-
Svensäter, G (författare)
-
visa färre...
-
(creator_code:org_t)
- Wiley, 2007
- 2007
- Engelska.
-
Ingår i: International endodontic journal. - : Wiley. - 0143-2885 .- 1365-2591. ; 40:5, s. 344-55
- Relaterad länk:
-
https://gup.ub.gu.se...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- AIM: To determine whether bacteria isolated from infected root canals survive alkaline shifts better in biofilms than in planktonic cultures. METHODOLOGY: Clinical isolates of Enterococcus faecalis, Lactobacillus paracasei, Olsenella uli, Streptococcus anginosus, S. gordonii, S. oralis and Fusobacterium nucleatum in biofilm and planktonic cultures were stressed at pH 10.5 for 4 h, and cell viability determined using the fluorescent staining LIVE/DEAD BacLight bacterial viability kit. In addition, proteins released into extracellular culture fluids were identified by Western blotting. RESULTS: Enterococcus faecalis, L. paracasei, O. uli and S. gordonii survived in high numbers in both planktonic cultures and in biofilms after alkaline challenge. S. anginosus, S. oralis and F. nucleatum showed increased viability in biofilms compared with planktonic cultures. Alkaline exposure caused all planktonic cultures to aggregate into clusters and resulted in a greater extrusion of cellular proteins compared with cells in biofilms. Increased levels of DnaK, HPr and fructose-1,6-bisphosphate aldolase were observed in culture fluids, especially amongst streptococci. CONCLUSIONS: In general, bacteria isolated from infected roots canals resisted alkaline stress better in biofilms than in planktonic cultures, however, planktonic cells appeared to use aggregation and the extracellular transport of specific proteins as survival mechanisms.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Odontologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dentistry (hsv//eng)
Nyckelord
- Animals
- Bacteria
- drug effects
- genetics
- Biofilms
- drug effects
- Calcium Hydroxide
- pharmacology
- Colony Count
- Microbial
- Dental Pulp Cavity
- microbiology
- Hydrogen-Ion Concentration
- Root Canal Irrigants
- pharmacology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas