Protein expression patterns associated with progression of chronic obstructive pulmonary disease in bronchoalveolar lavage of smokers

Plymoth, AmelieLund University,Lunds universitet,Lungmedicin, allergologi och palliativ medicin,Sektion II,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Respiratory Medicine, Allergology, and Palliative Medicine,Section II,Department of Clinical Sciences, Lund,Faculty of Medicine (författare)

BACKGROUND: We modeled the expression of proteins in baseline bronchoalveolar lavage (BAL) samples from asymptomatic 60-year-old lifelong current smokers or healthy never-smokers, who were reevaluated after 6 to 7 years to record clinical outcome. METHODS: Applying a technology toolbox consisting of replicate 2-dimensional gel separations, image annotation, and mass spectrometry identification, we catalogued a global set of proteins that were differentially expressed in individuals by presence, absence, and intensity scores. RESULTS: By use of multivariate analysis, we selected a subset of proteins that accurately separated smokers from never-smokers based on composite scoring. Follow-up after 6 to 7 years identified a group of individuals who had progressed to chronic obstructive pulmonary disease (COPD), Global Initiative for Chronic Obstructive Lung Disease stage 2. The baseline BAL samples of these eventual COPD patients shared a distinct protein expression profile that could be identified using partial least-squares discriminant analysis. This pattern was not observed in BAL samples of asymptomatic smokers free of COPD at 6- to 7-year follow-up. CONCLUSIONS: Our model suggests that certain patterns of protein expression occurring in the airways of long-term smokers may be detected in smokers susceptible to a progression of COPD disease, before disease is clinically evident.

Löfdahl, Claes-GöranLund University,Lunds universitet,Lungmedicin, allergologi och palliativ medicin,Sektion II,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Respiratory Medicine, Allergology, and Palliative Medicine,Section II,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)lung-cgl (författare)
Ekberg-Jansson, Ann,1960Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin,Institute of Medicine, Department of Internal Medicine(Swepub:gu)xekban (författare)
Dahlback, M. (författare)
Broberg, P. (författare)
Foster, M. (författare)
Fehniger, T. E. (författare)
Marko-Varga, GyörgyLund University,Lunds universitet,Centrum för analys och syntes,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Centre for Analysis and Synthesis,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)akem-gmv (författare)
Lungmedicin, allergologi och palliativ medicinSektion II (creator_code:org_t)

Ingår i:Clin Chem: Oxford University Press (OUP)53:4, s. 636-440009-91471530-8561
Ingår i:Clinical Chemistry: Oxford University Press (OUP)53:4, s. 636-441530-8561

Av författaren/redakt...: Plymoth, Amelie; Löfdahl, Claes-G ...; Ekberg-Jansson, ...; Dahlback, M.; Broberg, P.; Foster, M.; visa fler...; Fehniger, T. E.; Marko-Varga, Gyö ...; visa färre...

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