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Identification of t...
Identification of two isoforms of Atlantic halibut insulin-like growth factor-I receptor genes and quantitative gene expression during metamorphosis
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- Hildahl, Jon, 1976 (författare)
- Gothenburg University,Göteborgs universitet,Zoologiska institutionen,Department of Zoology
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Galay-Burgos, M (författare)
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Sweeney, G (författare)
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- Einarsdottir, Ingibjörg, 1951 (författare)
- Gothenburg University,Göteborgs universitet,Zoologiska institutionen,Department of Zoology
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- Björnsson, Björn Thrandur, 1952 (författare)
- Gothenburg University,Göteborgs universitet,Zoologiska institutionen,Department of Zoology
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(creator_code:org_t)
- Elsevier BV, 2007
- 2007
- Engelska.
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Ingår i: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. - : Elsevier BV. - 1096-4959. ; 147:3, s. 395-401
- Relaterad länk:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Insulin-like growth factor-I (IGF-I) is an important regulator of growth and development in vertebrates. Both the endocrine and paracrine actions of IGF-I are mediated through ligand-binding to a membrane-bound IGF-I receptor (IGF-IR). The characterization of this receptor and subsequent expression studies thus help elucidate the endocrine regulation of developmental processes. As other flatfish species, the Atlantic halibut (Hippoglossus hippoglossus) undergoes a dramatic larval metamorphosis. This process is largely under endocrine control, and data indicate that IGF-I could be a key regulator. IGF-I content increases up to late pre-metamorphosis and decreases during metamorphosis. The IGF-IR has, however, not been studied during flatfish metamorphosis. To examine IGF-IR gene expression, two IGF-IR mRNA were cloned and sequenced. These partial sequences share high identity (≥ 95%) and similarity (≥ 97%) with other fish IGF-IR and lower identity (≥ 77%) and similarity (≥ 83.5%) with Japanese flounder insulin receptors. The expression of mRNA for both IGF-IR was analyzed by quantitative real-time RT-PCR during six larval developmental stages from pre- to post-metamorphosis. IGF-IR1 and IGF-IR2 mRNA are differentially expressed during metamorphosis, but if this indicates an isoform-specific regulation of developmental processes by circulating and/or locally-secreted IGF-I is unclear. Both IGF-IR genes are down-regulated in halibut larvae experiencing arrested metamorphosis, suggesting the IGF-I system is critical for metamorphic success in halibut.
Ämnesord
- NATURVETENSKAP -- Biologi -- Zoologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Zoology (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Nyckelord
- Atlantic halibut
- Development
- Hippoglossus hippoglossus
- IGF-I
- IGF-R
- Metamorphosis
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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