High Osmolarity and L-DOPA Augment Release via the Fusion Pore in PC12 Cells

We have amperometrically measured dopamine release from rat pheochromocytoma cells (PC12 cells) in high osmolarity conditions with and without L-3,4-dihydroxyphenylalanine (L-DOPA) treatment. We observe an increase in the number of release events displaying a prespike feature or foot when the cells are stimulated in high osmolarity saline. We also see an increase in foot area and duration when cells are stimulated in high osmolarity saline, or high osmolarity saline subsequent to incubation with the dopamine precursor L-DOPA in isotonic saline, which serves to increase the vesicle size. The data suggest that membrane biophysics are an important component in defining the rate, duration and amount of neurotransmitter release via the fusion pore.

NATURVETENSKAP Kemi hsv//swe
NATURAL SCIENCES Chemical Sciences hsv//eng
amperometry • catecholamines • fusion pores • hyperosmotic solutions • membranes
amperometry • catecholamines • fusion pores • hyperosmotic solutions • membranes

Maxson, M M (författare)
Wittenberg, N J (författare)
Ewing, Andrew G,1957Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg(Swepub:cth)andrewe (författare)
Göteborgs universitetInstitutionen för kemi (creator_code:org_t)

Av författaren/redakt...: Sombers, L A; Maxson, M M; Wittenberg, N J; Ewing, Andrew G, ...

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