Cleavage of integrin by mu-calpain during hypoxia in human endometrial cells

PROBLEM: The distribution and activation of mu-calpain and possible cleavage of integrin in human endometrial cells under hypoxic condition were investigated. METHOD OF STUDY: Human endometrial epithelial and stromal cells were subjected to hypoxia, and subsequently used for immunostaining and western blot analysis. RESULTS: The proform of mu-calpain was detected in the cytoplasm of normal cells, and displayed a substantial decrease after hypoxia. Conversely, the active form of mu-calpain was not detected in normal cells, but was abundant after hypoxia. The cytoplasmic domain of integrin beta3 was also detected in the cytoplasm of endometrial cells. Western blot analysis confirmed that both the proform of mu-calpain and the integrin beta3 cytoplasmic domain decreased during hypoxia. CONCLUSIONS: Mu-calpain is activated in human endometrial cells during hypoxia and that subsequent cleavage of the integrin beta3 cytoplasmic domain may give some adverse effects to the function of human endometrium.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
Blotting
Western
Calpain/*metabolism
Cell Hypoxia/*physiology
Cell Survival
Cells
Cultured
Electrophoresis
Polyacrylamide Gel
Endometrium/*cytology/*metabolism
Female
Humans
Immunohistochemistry
Integrin beta3/*metabolism
Microscopy
Confocal

Ozaki, Y. (author)
Nakanishi, T. (author)
Ogasawara, M. S. (author)
Ikuta, K. (author)
Aoki, K. (author)
Blomgren, Klas,1963Gothenburg University,Göteborgs universitet,Institutionen för kvinnors och barns hälsa, Avdelningen för pediatrik,Institutionen för fysiologi och farmakologi, Avdelningen för fysiologi,Institute for the Health of Women and Children, Dept of Paediatrics,Institute of Physiology and Pharmacology, Dept of Physiology(Swepub:gu)xblokl (author)
Suzumori, K. (author)
Göteborgs universitetInstitutionen för kvinnors och barns hälsa, Avdelningen för pediatrik (creator_code:org_t)

By the author/editor: Aoyama, K.; Ozaki, Y.; Nakanishi, T.; Ogasawara, M. S.; Ikuta, K.; Aoki, K.; show more...; Blomgren, Klas, ...; Suzumori, K.; show less...

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MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

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