SwePub
Sök i LIBRIS databas

  Extended search

WFRF:(Neutze Richard)
 

Search: WFRF:(Neutze Richard) > A Lipidic-Sponge Ph...

  • Wöhri, Annemarie,1976Chalmers tekniska högskola,Chalmers University of Technology (author)

A Lipidic-Sponge Phase Screen for Membrane Protein Crystallization

  • Article/chapterEnglish2008

Publisher, publication year, extent ...

  • Elsevier BV,2008

Numbers

  • LIBRIS-ID:oai:gup.ub.gu.se/72384
  • https://gup.ub.gu.se/publication/72384URI
  • https://doi.org/10.1016/j.str.2008.06.003DOI
  • https://research.chalmers.se/publication/72384URI

Supplementary language notes

  • Language:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • A major current deficit in structural biology is the lack of high-resolution structures of eukaryotic membrane proteins, many of which are key drug targets for the treatment of disease. Numerous eukaryotic membrane proteins require specific lipids for their stability and activity, and efforts to crystallize and solve the structures of membrane proteins that do not address the issue of lipids frequently end in failure rather than success. To help address this problem, we have developed a sparse matrix crystallization screen consisting of 48 lipidic-sponge phase conditions. Sponge phases form liquid lipid bilayer environments which are suitable for conventional hanging- and sitting-drop crystallization experiments. Using the sponge phase screen, we obtained crystals of several different membrane proteins from bacterial and eukaryotic sources. We also demonstrate how the screen may be manipulated by incorporating specific lipids such as cholesterol; this modification led to crystals being recovered from a bacterial photosynthetic core complex.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Johansson, Linda C,1983Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg(Swepub:gu)xjlinf (author)
  • Wadsten-Hindrichsen, Pia,1969 (author)
  • Wahlgren, Weixiao Yuan,1970Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg(Swepub:gu)xyuawe (author)
  • Fischer, Gerhard,1978Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg(Swepub:gu)xfisge (author)
  • Horsefield, Rob,1977Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg(Swepub:gu)xhorro (author)
  • Katona, Gergely,1975Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg(Swepub:gu)xkatge (author)
  • Nyblom, Anna Maria,1975Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)mbtkmn (author)
  • Öberg, Fredrik,1982Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg(Swepub:gu)xobefr (author)
  • Young, G (author)
  • Cogdell, Richard (author)
  • Fraser, N J (author)
  • Engström, Sven,1951Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)sv (author)
  • Neutze, Richard,1969Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg(Swepub:cth)xneuri (author)
  • Hindrichsen, Pia Jeanette,1969Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)wadsten (author)
  • Chalmers tekniska högskolaInstitutionen för kemi (creator_code:org_t)

Related titles

  • In:Structure: Elsevier BV16:7, s. 1003-10090969-21261878-4186

Internet link

Find in a library

  • Structure (Search for host publication in LIBRIS)

To the university's database

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view