Functional expression of mu-opioid receptors in the human colon cancer cell line, HT-29, and their localization in human colon.

Nylund, Gunnar,1959Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences (author)

We have investigated the functional expression of mu-opioid receptors (MORs) in the human colon cancer cell line, HT-29. As revealed by immunocytochemistry, immunoreactivity was present in both the cytoplasm and nuclei of the cells. Challenge with morphine for 24 h (1 nM to 1 microM) barely affected cell proliferation, while the secretion of urokinase type plasminogen activator (a protease involved in invasion/metastasis) was markedly augmented by a concentration of 0.1 microM. Human colon cancer tissue from 14 consecutively operated patients was investigated by immunohistochemistry. MORs were found in the nuclei of colonocytes and immune cells of the lamina propria in tumor-free tissue. In tumor tissue, immunoreactivity was found in the membrane and often in the nuclei of tumor cells. The current findings suggest that morphine administration could affect tumor progression by interfering with, for example, invasive properties. Our demonstration of a nuclear expression of the MORs appears to be a novel finding.

Pettersson, Ann,1982-Högskolan i Kalmar,Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences,Naturvetenskapliga institutionen,Department of Surgery, Sahlgrenska University Hospital, Göteborg, SE-413 45, Sweden(Swepub:lnu)fpean (author)
Bengtsson, Cecilia,1980Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences(Swepub:gu)xbecec (author)
Khorram-Manesh, Amir,1958Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences(Swepub:gu)xkhoam (author)
Nordgren, Svante,1945Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences(Swepub:gu)xnorsv (author)
Delbro, Dick,1950Högskolan i Kalmar,Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences,Naturvetenskapliga institutionen,Department of Surgery, Sahlgrenska University Hospital, Göteborg, SE-413 45, Sweden(Swepub:lnu)ndedi (author)
Göteborgs universitetInstitutionen för kliniska vetenskaper (creator_code:org_t)

In:Digestive diseases and sciences: Springer Science and Business Media LLC53:2, s. 461-60163-21161573-2568

By the author/editor: Nylund, Gunnar, ...; Pettersson, Ann, ...; Bengtsson, Cecil ...; Khorram-Manesh, ...; Nordgren, Svante ...; Delbro, Dick, 19 ...

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.