Estrogen receptor specificity for the effects of estrogen in ovariectomized mice.

• Estrogen exerts a variety of important physiological effects, which have been suggested to be mediated via the two known estrogen receptors (ERs), alpha and beta. Three-month-old ovariectomized mice, lacking one or both of the two estrogen receptors, were given estrogen subcutaneously (2.3 micro g/mouse per day) and the effects on different estrogen-responsive parameters, including skeletal effects, were studied. We found that estrogen increased the cortical bone dimensions in both wild-type (WT) and double ER knockout (DERKO) mice. DNA microarray analysis was performed to characterize this effect on cortical bone and it identified four genes that were regulated by estrogen in both WT and DERKO mice. The effect of estrogen on cortical bone in DERKO mice might either be due to remaining ERalpha activity or represent an ERalpha/ERbeta-independent effect. Other effects of estrogen, such as increased trabecular bone mineral density, thymic atrophy, fat reduction and increased uterine weight, were mainly ERalpha mediated.

Nyckelord

Animals

Bone Density

drug effects

Bone and Bones

drug effects

Collagen Type VIII

genetics

Complement C3

genetics

Estrogen Receptor alpha

Estrogen Receptor beta

Estrogens

pharmacology

Female

Interleukin-3

genetics

Liver

drug effects

Matrix Metalloproteinase 9

genetics

Mice

Mice

Knockout

Organ Size

drug effects

Ovariectomy

RNA

Messenger

analysis

Receptors

Estrogen

genetics

physiology

Sialoglycoproteins

genetics

Thymus Gland

cytology

Uterus

cytology

metabolism

alpha-Macroglobulins

genetics

Mice

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)