Metalloid tolerance based on phytochelatins is not functionally equivalent to the arsenite transporter Acr3p.

Active transport of metalloids by Acr3p and Ycf1p in Saccharomyces cerevisiae and chelation by phytochelatins in Schizosaccharomyces pombe, nematodes, and plants represent distinct strategies of metalloid detoxification. In this report, we present results of functional comparison of both resistance mechanisms. The S. pombe and wheat phytochelatin synthase (PCS) genes, when expressed in S. cerevisiae, mediate only modest resistance to arsenite and thus cannot functionally compensate for Acr3p. On the other hand, we show for the first time that phytochelatins also contribute to antimony tolerance as PCS fully complement antimonite sensitivity of ycf1Delta mutant. Remarkably, heterologous expression of PCS sensitizes S. cerevisiae to arsenate, while ACR3 confers much higher arsenic resistance in pcsDelta than in wild-type S. pombe. The analysis of PCS and ACR3 homologues distribution in various organisms and our experimental data suggest that separation of ACR3 and PCS genes may lead to the optimal tolerance status of the cell.

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
Aminoacyltransferases
classification
genetics
physiology
Antimony
pharmacology
Arsenic
pharmacology
Arsenites
metabolism
Biological Transport
Active
Drug Resistance
Fungal
Glutathione
Membrane Proteins
classification
physiology
Membrane Transport Proteins
classification
physiology
Metalloproteins
physiology
Metals
pharmacology
Mutation
Phylogeny
Phytochelatins
Saccharomyces cerevisiae
drug effects
enzymology
metabolism
Saccharomyces cerevisiae Proteins
Schizosaccharomyces
drug effects
enzymology
metabolism
Yeasts
drug effects

Clemens, Stephan (författare)
Augustyniak, Daria (författare)
Golik, Pawel (författare)
Maciaszczyk, Ewa (författare)
Tamás, Markus J.,1970Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology(Swepub:gu)xtamma (författare)
Dziadkowiec, Dorota (författare)
Göteborgs universitetInstitutionen för cell- och molekylärbiologi (creator_code:org_t)

Ingår i:Biochemical and biophysical research communications304:2, s. 293-3000006-291X

Av författaren/redakt...: Wysocki, Robert; Clemens, Stephan; Augustyniak, Dar ...; Golik, Pawel; Maciaszczyk, Ewa; Tamás, Markus J. ...; visa fler...; Dziadkowiec, Dor ...; visa färre...

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