Bromodeoxyuridine and the detection of neurogenesis.

• Bromodeoxyuridine (BrdU) is widely used for labeling dividing cells to determine their fate. In particular, the analysis of neurogenesis in the adult mammalian brain has made significant progress through the use of this technique. However; when using BrdU for labeling, there are several issues to consider in order to minimalize possible cytotoxicity or false-positive labeling. This current review summarizes methodological and technical aspects of BrdU administration and detection, compares alternative methods and gives recommendations on how to avoid labeling artifacts.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)

Nyckelord

Animals

Artifacts

Bromodeoxyuridine

analysis

Cell Proliferation

Cells

Cultured

Humans

Image Enhancement

methods

Microscopy

Fluorescence

methods

Nerve Regeneration

physiology

Neurons

cytology

drug effects

physiology

Staining and Labeling

methods

Publikations- och innehållstyp

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