WFRF:(Rydberg Lennart 1944)
Sökning: WFRF:(Rydberg Lennart 1944) > Studies on glycolip...
- Diswall, Mette,1979Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences (författare)
Studies on glycolipid antigens in small intestine and pancreas from alpha1,3-galactosyltransferase knockout miniature swine.
- Artikel/kapitelEngelska2007
Förlag, utgivningsår, omfång ...
- Ovid Technologies (Wolters Kluwer Health),2007
Nummerbeteckningar
- LIBRIS-ID:oai:gup.ub.gu.se/81224
- https://gup.ub.gu.se/publication/81224URI
- https://doi.org/10.1097/01.tp.0000287599.46165.15DOI
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- Språk:engelska
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- BACKGROUND: To avoid hyperacute rejection of xeno-organs, alpha1,3-galactosyltransferase knockout (GalT-KO) pigs have been produced. Galalpha1,3Gal determinant elimination may expose cryptic carbohydrate antigens and/or generate new antigens. This is the first biochemical study of carbohydrate antigens in GalT-KO pig organs. METHODS: Neutral and acidic glycolipids were isolated from small intestine and pancreas of two GalT-KO and one wild-type (WT) pig. Glycolipid immune reactivity was tested on thin-layer chromatograms. Small intestine neutral glycolipids were separated by high-performance liquid chromatography and selected fractions were analyzed by proton nuclear magnetic resonance spectroscopy. Total gangliosides were quantified on thin-layer chromatograms and in microtiter wells. RESULTS: Using Galalpha1,3nLc4 glycolipid reference, total Galalpha1,3Gal glycolipid antigens in the WT animal was estimated at about 30 microg (small intestine) and 3 microg (pancreas) per gram of dry tissue. Galalpha1,3Gal determinants were not detected in GalT-KO tissues at a detection limit of less than 0.25% (small intestine) and 0.5% (pancreas) of the WT tissues. Isoglobotriaosylceramide (iGb3) was absent but trace amounts of Fuc-iGb3 was found in both GalT-KO and WT pig small intestine. Blood group H type 2 core saccharide compounds were increased in GalT-KO pancreas. Total amount of gangliosides was decreased in GalT-KO tissues. The alpha1,3-galactosyltransferase acceptor, N-acetyllactosamine determinant, was not increased in GalT-KO tissues. Human serum antibodies reacted with WT organ Galalpha1,3Gal antigens and gangliosides, of which the ganglioside reactivity remained in GalT-KO tissues. CONCLUSIONS: Knockout of porcine alpha1,3-galactosyltransferase gene results in elimination of Galalpha1,3Gal-terminated glycolipid compounds. GalT-KO genetic modification did not produce new compensatory glycolipid compounds reactive with human serum antibodies.
Ämnesord och genrebeteckningar
- Animals
- Animals
- Genetically Modified
- Antibodies
- blood
- Antigens
- analysis
- Galactosyltransferases
- deficiency
- genetics
- Glycolipids
- analysis
- immunology
- Humans
- Intestine
- Small
- immunology
- Pancreas
- immunology
- Swine
- Swine
- Miniature
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Ångström, Jonas,1950Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences(Swepub:gu)xangjo (författare)
- Dor, Frank J M F (författare)
- Schuurman, Henk-Jan (författare)
- Rydberg, Lennart,1944Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine(Swepub:gu)xrydle (författare)
- Breimer, Michael,1951Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences(Swepub:gu)xbremi (författare)
- Göteborgs universitetInstitutionen för kliniska vetenskaper (creator_code:org_t)
Sammanhörande titlar
- Ingår i:Transplantation: Ovid Technologies (Wolters Kluwer Health)84:10, s. 1348-560041-1337
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- Diswall, Mette, ...
- Ångström, Jonas, ...
- Dor, Frank J M F
- Schuurman, Henk- ...
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- Breimer, Michael ...
- Artiklar i publikationen
- Transplantation
- Av lärosätet
- Göteborgs universitet
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