Cell death-inducing DFF45-like effector C is reduced by caloric restriction and regulates adipocyte lipid metabolism.

• Members of the cell death-inducing DFF45-like effector (CIDE) gene family have been shown to regulate lipid metabolism. In this article, we report that the third member of the human CIDE family, CIDEC, is down-regulated in response to a reduced caloric intake. The down-regulation was demonstrated by microarray and real-time polymerase chain reaction analysis of subcutaneous adipose tissue in 2 independent studies on obese patients undergoing treatment with a very low calorie diet. By analysis of CIDEC expression in 65 human tissues, we conclude that human CIDEC is predominantly expressed in subcutaneous adipocytes. Together, these observations led us to investigate the effect of decreased CIDEC expression in cultured 3T3-L1 adipocytes. Small interfering RNA-mediated knockdown of CIDEC resulted in an increased basal release of nonesterified fatty acids, decreased responsiveness to adrenergic stimulation of lipolysis, and increased oxidation of endogenous fatty acids. Thus, we suggest that CIDEC is a regulator of adipocyte lipid metabolism and may be important for the adipocyte to adapt to changes in energy availability.

Nyckelord

3T3-L1 Cells

Adipocytes

drug effects

metabolism

Adrenergic beta-Agonists

pharmacology

Adult

Animals

Caloric Restriction

Down-Regulation

Fatty Acids

metabolism

Fatty Acids

Nonesterified

metabolism

Female

Gene Silencing

Humans

Isoproterenol

pharmacology

Lipid Metabolism

Lipolysis

drug effects

Male

Mice

Middle Aged

Oxidation-Reduction

Protein Isoforms

metabolism

Proteins

genetics

metabolism

RNA

Small Interfering

pharmacology

Tissue Distribution

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)