Sublingual immunization induces broad-based systemic and mucosal immune responses in mice.

The potential of sublingual (s.l.) delivery of vaccine was examined in mice. We show the existence of a dense network of dendritic cells (DCs) in the s.l. epithelium and a rapid and transient increase in the frequency of s.l. DCs after topical application of cholera toxin (CT) adjuvant under the tongue. S.l. immunization with ovalbumin and CT induced vigorous systemic and mucosal antibody responses. Such treatment promoted mixed Th1 and Th2 cytokine responses and induced cytotoxic CD8(+) T cells in lung tissues and in systemic lymphoid organs. S.l. immunization was comparable to intranasal immunization and was superior to oral immunization regarding the magnitude and anatomic dissemination of the induced immune responses. S.l. administration of live influenza virus at a dose lethal by the nasal route was well tolerated and did not redirect virus to the olfactory bulb. These features underscore the potential of the s.l. mucosa to serve as an alternative vaccine delivery route.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Mikrobiologi inom det medicinska området hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Microbiology in the medical area hsv//eng
Adjuvants
Immunologic
Administration
Sublingual
Adoptive Transfer
Animals
Antibodies
Viral
analysis
biosynthesis
Antibody-Producing Cells
CD4-Positive T-Lymphocytes
immunology
CD8-Positive T-Lymphocytes
immunology
Cholera Toxin
immunology
Cytokines
biosynthesis
Dendritic Cells
immunology
Dose-Response Relationship
Immunologic
Female
Flow Cytometry
Immunity
Mucosal
immunology
Immunization
methods
Immunohistochemistry
Mice
Mice
Inbred BALB C
Mice
Inbred C57BL
Mouth Mucosa
cytology
immunology
Ovalbumin
immunology
Reverse Transcriptase Polymerase Chain Reaction

Kweon, Mi-Na (author)
Song, Joo-Hye (author)
Hervouet, Catherine (author)
Luci, Carmelo (author)
Sun, Jia-Bin,1948Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology(Swepub:gu)xsunji (author)
Hofman, Paul (author)
Holmgren, Jan,1944Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology(Swepub:gu)xholja (author)
Anjuère, Fabienne (author)
Czerkinsky, Cecil (author)
Göteborgs universitetInstitutionen för biomedicin, avdelningen för mikrobiologi och immunologi (creator_code:org_t)

By the author/editor: Cuburu, Nicolas; Kweon, Mi-Na; Song, Joo-Hye; Hervouet, Cather ...; Luci, Carmelo; Sun, Jia-Bin, 19 ...; show more...; Hofman, Paul; Holmgren, Jan, 1 ...; Anjuère, Fabienn ...; Czerkinsky, Ceci ...; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Microbiology in ...

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