Genetic analyses of the role of RCE1 in RAS membrane association and transformation.

• Proteins terminating with a CAAX motif, such as the nuclear lamins and the RAS family of proteins, undergo post-translational modification of a carboxyl-terminal cysteine with an isoprenyl lipid--a process called protein prenylation. After prenylation, the last three residues of CAAX proteins are clipped off by an endoprotease of the endoplasmic reticulum. RCE1 is responsible for the endoproteolytic processing of the RAS proteins and is likely responsible for endoproteolytic processing of the vast majority of CAAX proteins. Prenylation has been shown to be essential for the proper intracellular targeting and function of several CAAX proteins, but the physiologic importance of the endoprotease step has remained less certain. Here, we will review methods that have been used to define the physiologic importance of the endoproteolytic processing step of CAAX protein processing.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)

Keyword

Animals

Cardiomyopathy

Dilated

etiology

Cell Proliferation

Cell Transformation

Neoplastic

Cells

Cultured

Electrophoresis

Polyacrylamide Gel

Endopeptidases

deficiency

genetics

physiology

Fibroblasts

metabolism

Genes

ras

Hematopoiesis

physiology

Humans

Liver

physiology

Methylation

Mice

Mice

Knockout

Mice

Nude

Mitogen-Activated Protein Kinase 1

metabolism

Mitogen-Activated Protein Kinase 3

metabolism

Myeloproliferative Disorders

etiology

Protein Processing

Post-Translational

Transfection

ras Proteins

isolation & purification

metabolism

Publication and Content Type

ref (subject category)

art (subject category)