WFRF:(Ohlsson Claes J.)
Sökning: WFRF:(Ohlsson Claes J.) > (1995-1999) > Growth hormone rece...
- Slootweg, M C (författare)
Growth hormone receptor activity is stimulated by insulin-like growth factor binding protein 5 in rat osteosarcoma cells.
- Artikel/kapitelEngelska1996
Förlag, utgivningsår, omfång ...
- 1996
Nummerbeteckningar
- LIBRIS-ID:oai:gup.ub.gu.se/91469
- https://gup.ub.gu.se/publication/91469URI
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- Språk:engelska
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- SwePubSwePub
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- Osteoblast-like UMR-106.01 rat osteosarcoma cells express high affinity growth hormone (GH) receptors (GHRs). Because osteoblasts secrete insulin-like growth factor binding protein-5 (IGFBP-5), we evaluated whether it also modulates GH binding and GHR expression in UMR cells. Human recombinant intact IGFBP-5 stimulated 125I-hGH binding in a dose-dependent manner (dose range 300-3000 ng/ml), inducing an increase to 193.6 +/- 2.1% of control binding at 3000 ng/ml (P < 0.001). Carboxy-truncated IGFBP-5 also stimulated GH binding but with less potency (125 +/- 2.7% of control at 3000 ng/ml, P < 0.01). GHRs identified by chemical crosslinking of 125I-hGH to cell monolayers increased after treatment with IGFBP-5 and decreased in response to insulin-like growth factor-I (IGF-I). GHR mRNA levels, as quantitated by a solution hybridization RNAse protection assay, increased up to 3 to 7-fold in a time-dependent manner by intact IGFBP-5 but not by carboxy-truncated IGFBP-5. An antiserum to IGFBP-5 reduced basal GH binding to 56.7 +/- 4.3% of control value at a concentration of 0.5% (P < 0.001), showing that IGFBP-5 produced by the cells is a strong regulator of GH binding. IGFBP-5 antiserum also decreased GH binding to 85.9 +/- 0.9% of IGFBP-5 stimulated value (P < 0.001), showing the specificity of IGFBP-5 stimulation. To determine whether the GHR upregulation was physiologically significant, cell proliferation was evaluated after coincubation of IGFBP-5 with low, non-stimulatory concentrations of GH. IGFBP-5 (1000 ng/ml) induced cell proliferation to 116.2 +/- 3.2% of control levels, and coincubation with hGH at 10 ng/ml induced an increase to 133.3 +/- 0.1% of control levels. We conclude that exogenous and endogenous IGFBP-5 upregulate GHR mRNA levels and GH binding and this interaction potentiates GH-stimulated mitogenesis in osteoblastic cells.
Ämnesord och genrebeteckningar
- Animals
- Bone Neoplasms
- metabolism
- Cell Division
- drug effects
- Cell Line
- Cross-Linking Reagents
- Growth Hormone
- pharmacology
- Humans
- Insulin-Like Growth Factor Binding Protein 5
- pharmacology
- Iodine Radioisotopes
- diagnostic use
- Isotope Labeling
- Osteosarcoma
- metabolism
- Radioligand Assay
- Rats
- Receptors
- Somatotropin
- drug effects
- Ribonucleases
- metabolism
- Tumor Cells
- Cultured
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Ohlsson, Claes,1965Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för internmedicin,Institute of Internal Medicine, Dept of Medicine(Swepub:gu)xohlcl (författare)
- van Elk, E J (författare)
- Netelenbos, J C (författare)
- Andress, D L (författare)
- Göteborgs universitetInstitutionen för invärtesmedicin, Avdelningen för internmedicin (creator_code:org_t)
Sammanhörande titlar
- Ingår i:Growth regulation6:4, s. 238-460956-523X
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- Slootweg, M C
- Ohlsson, Claes, ...
- van Elk, E J
- Netelenbos, J C
- Andress, D L
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- Growth regulatio ...
- Av lärosätet
- Göteborgs universitet
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