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The application and...
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Fredin, Maria Fritsch,1970Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology,Astra-Zeneca, Mölndal
(author)
The application and relevance of ex vivo culture systems for assessment of IBD treatment in murine models of colitis
- Article/chapterEnglish2008
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Numbers
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LIBRIS-ID:oai:gup.ub.gu.se/96675
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https://gup.ub.gu.se/publication/96675URI
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https://doi.org/10.1016/j.phrs.2008.08.006DOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-6102URI
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-125820URI
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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The aim of this study was to investigate the relevance of mouse ex vivo cultures as a first screening model for new therapeutic agents of Inflammatory Bowel Disease (IBD). Two murine models (dextran sodium sulphate (DSS)-induced colitis and Gαi2-deficient mice) and two anti-inflammatory agents (methyl-prednisolone and the proteasome inhibitor MG132) were evaluated. The in vivo effects of methyl-prednisolone were assessed in both models. Ex vivo colonic tissue from both mouse models were cultured in the presence or absence of the drugs and TaqMan Low-Density arrays were used to assess the regulation of inflammatory genes before and after drug treatment. Colitis induced a similar inflammatory gene profile in both mouse models in in vivo studies and in ex vivo cultures. The differences encountered reflected the different phases of colitis in the models, e.g. innate cytokine/chemokine profile in the DSS model and T cell related markers in Gαi2-deficient mice. After steroid treatment, a similar pattern of genes was suppressed in the two mouse models. We confirmed the suppression of inflammatory gene expression for IL-1β, IL-6 and iNOS in ex vivo and in vivo colons from both mouse models by quantitative RT-PCR. Importantly, the inflammatory responses in the murine ex vivo culture system reflected the in vivo response in the inflamed colonic tissue as assessed by changes in inflammatory gene expression, suggesting that the murine culture system can be used for validation of future IBD therapies.
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Vidal, AlexanderAstra-Zeneca, Mölndal
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Utkovic, HelenaAstra-Zeneca, Mölndal
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Götlind, Yu-Yuan Chiu,1964Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology,Inst. för Biomedicin, Göteborgs Universitet(Swepub:gu)xgotyu
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Willen, RogerUppsala universitet,Institutionen för genetik och patologi
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Jansson, LiselotteAstra-Zeneca, Mölndal
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Hultgren-Hörnquist, Elisabeth,1965Örebro universitet,Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology,Hälsoakademin(Swepub:oru)eht
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Melgar, SilviaAstra-Zeneca, Mölndal
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Göteborgs universitetInstitutionen för biomedicin, avdelningen för mikrobiologi och immunologi
(creator_code:org_t)
Related titles
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In:Pharmacological Research: Elsevier BV58:3-4, s. 222-2311043-66181096-1186
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