Search: LAR1:gu
> (2000-2009) >
Activity of the sma...
Activity of the small modified amino acid alpha-hydroxy glycineamide on in vitro and in vivo human immunodeficiency virus type 1 capsid assembly and infectivity
-
- Abdurahman, Samir, 1965- (author)
- Karolinska Institutet
-
- Vegvari, Akos (author)
- Clinical Protein Science, Department of Electrical Measurements, Lund University, Lund, Sweden
-
- Youssefi, Masoud (author)
- Karolinska Institutet
-
show more...
-
- Levi, Michael (author)
- Tripep AB, Huddinge, Sweden
-
- Höglund, Stefan (author)
- Uppsala universitet,Institutionen för biokemi och organisk kemi
-
- Andersson, Elin, 1975 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine,Department of Clinical Virology, University of Göteborg, Göteborg, Sweden
-
- Horal, Peter, 1955 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine,Department of Clinical Virology, University of Göteborg, Göteborg, Sweden
-
- Svennerholm, Bo, 1949 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine,Department of Clinical Virology, University of Göteborg, Göteborg, Sweden
-
- Balzarini, Jan (author)
- Institute for Medical Research, Katholieke Universiteit Leuven, Leuven, Belgium
-
- Vahlne, Anders (author)
- Karolinska Institutet
-
show less...
-
(creator_code:org_t)
- 2008
- 2008
- English.
-
In: Antimicrobial Agents and Chemotherapy. - 0066-4804 .- 1098-6596. ; 52:10, s. 3737-3744
- Related links:
-
https://gup.ub.gu.se...
-
show more...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
http://kipublication...
-
https://urn.kb.se/re...
-
show less...
Abstract
Subject headings
Close
- Upon maturation of the human immunodeficiency virus type 1 (HIV-1) virion, proteolytic cleavage of the Gag precursor protein by the viral protease is followed by morphological changes of the capsid protein p24, which will ultimately transform the virus core from an immature spherical to a mature conical structure. Virion infectivity is critically dependent on the optimal semistability of the capsid cone structure. We have reported earlier that glycineamide (G-NH(2)), when added to the culture medium of infected cells, inhibits HIV-1 replication and that HIV-1 particles with aberrant core structures were formed. Here we show that it is not G-NH(2) itself but a metabolite thereof, alpha-hydroxy-glycineamide (alpha-HGA), that is responsible for the antiviral activity. We show that alpha-HGA inhibits the replication of clinical HIV-1 isolates with acquired resistance to reverse transcriptase and protease inhibitors but has no effect on the replication of any of 10 different RNA and DNA viruses. alpha-HGA affected the ability of the HIV-1 capsid protein to assemble into tubular or core structures in vitro and in vivo, probably by binding to the hinge region between the N- and C-terminal domains of the HIV-1 capsid protein as indicated by matrix-assisted laser desorption ionization-mass spectrometry results. As an antiviral compound, alpha-HGA has an unusually simple structure, a pronounced antiviral specificity, and a novel mechanism of antiviral action. As such, it might prove to be a lead compound for a new class of anti-HIV substances.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmaceutiska vetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmaceutical Sciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
Keyword
- Anti-HIV Agents
- pharmacology
- Capsid Proteins
- physiology
- Drug Resistance
- Viral
- genetics
- Glycine
- analogs & derivatives
- pharmacology
- HIV Core Protein p24
- drug effects
- genetics
- HIV Infections
- drug therapy
- virology
- HIV-1
- drug effects
- genetics
- pathogenicity
- physiology
- Hela Cells
- Humans
- Microbial Sensitivity Tests
- Microscopy
- Electron
- Transmission
- Mutation
- Spectrometry
- Mass
- Matrix-Assisted Laser Desorption-Ionization
- Virulence
- drug effects
- Virus Assembly
- drug effects
- Virus Replication
- drug effects
- Mikrobiologi
- PHARMACY
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Abdurahman, Sami ...
-
Vegvari, Akos
-
Youssefi, Masoud
-
Levi, Michael
-
Höglund, Stefan
-
Andersson, Elin, ...
-
show more...
-
Horal, Peter, 19 ...
-
Svennerholm, Bo, ...
-
Balzarini, Jan
-
Vahlne, Anders
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Pharmaceutical S ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Microbiology in ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Pharmacology and ...
- Articles in the publication
-
Antimicrobial Ag ...
- By the university
-
University of Gothenburg
-
Örebro University
-
Karolinska Institutet
-
Uppsala University