Excitatory and inhibitory intermediate zone interneurons in pathways from feline group I and II afferents: differences in axonal projections and input.

• The aim of the present study was to compare properties of excitatory and inhibitory spinal intermediate zone interneurons in pathways from group I and II muscle afferents in the cat. Interneurons were labelled intracellularly and their transmitter phenotypes were defined by using immunocytochemistry. In total 14 glutamatergic, 22 glycinergic and 2 GABAergic/glycinergic interneurons were retrieved. All interneurons were located in laminae V-VII of the L3-L7 segments. No consistent differences were found in the location, the soma sizes or the extent of the dendritic trees of excitatory and inhibitory interneurons. However, major differences were found in their axonal projections; excitatory interneurons projected either ipsilaterally, bilaterally or contralaterally, while inhibitory interneurons projected exclusively ipsilaterally. Terminal projections of glycinergic and glutamatergic cells were found within motor nuclei as well as other regions of the grey matter which include the intermediate region, laminae VII and VIII. Cells containing GABA/glycine had more restricted projections, principally within the intermediate zone where they formed appositions with glutamatergic axon terminals and unidentified cells and therefore are likely to be involved in presynaptic as well as postsynaptic inhibition. The majority of excitatory and inhibitory interneurons were found to be coexcited by group I and II afferents (monosynaptically) and by reticulospinal neurons (mono- or disynaptically) and to integrate information from several muscles. Taken together the morphological and electrophysiological data show that individual excitatory and inhibitory intermediate zone interneurons may operate in a highly differentiated way and thereby contribute to a variety of motor synergies.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Physiology (hsv//eng)

Nyckelord

Afferent Pathways

cytology

physiology

Animals

Axons

metabolism

physiology

Carrier Proteins

metabolism

Cats

Choline O-Acetyltransferase

metabolism

Dendrites

metabolism

Electric Stimulation

Excitatory Postsynaptic Potentials

physiology

Glutamate Decarboxylase

metabolism

Inhibitory Postsynaptic Potentials

physiology

Interneurons

cytology

metabolism

physiology

Membrane Proteins

metabolism

Motor Neurons

cytology

metabolism

physiology

Muscle

Skeletal

innervation

Peripheral Nerves

physiology

Reflex

physiology

Spinal Cord

cytology

Vesicular Glutamate Transport Protein 1

metabolism

Vesicular Glutamate Transport Protein 2

metabolism

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)