Is acetylcholine an autocrine/paracrine growth factor via the nicotinic alpha7-receptor subtype in the human colon cancer cell line HT-29?

• We used immunochemistry to demonstrate expression of acetylcholine's nicotinic alpha7-receptor subtype in human colon cancer cell line HT-29. Moreover, RT-PCR and immunochemistry showed that choline acetyltransferase and acetylcholine esterase, the enzymes responsible for acetylcholine synthesis and degradation, respectively, localise in HT-29 cells. Bromoacetylcholine bromide, an inhibitor of choline acetyltransferase, significantly attenuated basal cell growth. Our findings suggest that acetylcholine might serve as an autocrine/paracrine-or speculatively, even intracrine-signalling molecule in cell line HT-29, thus contributing to carcinogenesis/cancer progression.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Kirurgi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Surgery (hsv//eng)

Nyckelord

Acetylcholine

biosynthesis

metabolism

Acetylcholinesterase

metabolism

Autocrine Communication

Biotinylation

Cell Line

Tumor

Choline O-Acetyltransferase

metabolism

Colonic Neoplasms

metabolism

Fluorescent Antibody Technique

Indirect

HT29 Cells

Humans

Immunohistochemistry

Intercellular Signaling Peptides and Proteins

metabolism

Paracrine Communication

Receptors

Nicotinic

metabolism

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