SwePub
Sök i LIBRIS databas

  Extended search

WFRF:(Ong Chee Bing)
 

Search: WFRF:(Ong Chee Bing) > Knockout of the non...

  • Niska-Blakie, JoannaA*Star Institute of Molecular and Cell Biology (IMCB),A*Star, Bioinformatics Institute (BII) (author)

Knockout of the non-essential gene SUGCT creates diet-linked, age-related microbiome disbalance with a diabetes-like metabolic syndrome phenotype

  • Article/chapterEnglish2019

Publisher, publication year, extent ...

  • 2019-11-13
  • Springer Science and Business Media LLC,2019

Numbers

  • LIBRIS-ID:oai:lup.lub.lu.se:005a2a53-8b9b-488c-9822-a2f219dfae6a
  • https://lup.lub.lu.se/record/005a2a53-8b9b-488c-9822-a2f219dfae6aURI
  • https://doi.org/10.1007/s00018-019-03359-zDOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:art swepub-publicationtype
  • Subject category:ref swepub-contenttype

Notes

  • SUGCT (C7orf10) is a mitochondrial enzyme that synthesizes glutaryl-CoA from glutarate in tryptophan and lysine catabolism, but it has not been studied in vivo. Although mutations in Sugct lead to Glutaric Aciduria Type 3 disease in humans, patients remain largely asymptomatic despite high levels of glutarate in the urine. To study the disease mechanism, we generated SugctKO mice and uncovered imbalanced lipid and acylcarnitine metabolism in kidney in addition to changes in the gut microbiome. After SugctKO mice were treated with antibiotics, metabolites were comparable to WT, indicating that the microbiome affects metabolism in SugctKO mice. SUGCT loss of function contributes to gut microbiota dysbiosis, leading to age-dependent pathological changes in kidney, liver, and adipose tissue. This is associated with an obesity-related phenotype that is accompanied by lipid accumulation in kidney and liver, as well as “crown-like” structures in adipocytes. Furthermore, we show that the SugctKO kidney pathology is accelerated and exacerbated by a high-lysine diet. Our study highlights the importance of non-essential genes with no readily detectable early phenotype, but with substantial contributions to the development of age-related pathologies, which result from an interplay between genetic background, microbiome, and diet in the health of mammals.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Gopinathan, LakshmiA*Star Institute of Molecular and Cell Biology (IMCB) (author)
  • Low, Kia NgeeA*Star, Bioinformatics Institute (BII) (author)
  • Kien, Yang LayA*Star, Bioinformatics Institute (BII) (author)
  • Goh, Christine M.F.A*Star Institute of Molecular and Cell Biology (IMCB) (author)
  • Caldez, Matias J.A*Star Institute of Molecular and Cell Biology (IMCB),National University of Singapore (author)
  • Pfeiffenberger, ElisabethA*Star Institute of Molecular and Cell Biology (IMCB) (author)
  • Jones, Oliver S.A*Star Institute of Molecular and Cell Biology (IMCB) (author)
  • Ong, Chee BingA*Star Institute of Molecular and Cell Biology (IMCB) (author)
  • Kurochkin, Igor V.A*Star, Bioinformatics Institute (BII) (author)
  • Coppola, VincenzoOhio State University (author)
  • Tessarollo, LinoNational Cancer Institute at Frederick (author)
  • Choi, HyungwonA*Star Institute of Molecular and Cell Biology (IMCB),National University of Singapore (author)
  • Kanagasundaram, YoganathanA*Star, Bioinformatics Institute (BII) (author)
  • Eisenhaber, FrankNanyang Technological University,A*Star, Bioinformatics Institute (BII) (author)
  • Maurer-Stroh, SebastianA*Star, Bioinformatics Institute (BII),National University of Singapore (author)
  • Kaldis, PhilippLund University,Lunds universitet,CRC, Clinical Research Centre,Medicinska fakulteten,Diabetiska komplikationer,Forskargrupper vid Lunds universitet,Metabolism och leversjukdomar,CRC, Clinical Research Centre,Faculty of Medicine,Diabetic Complications,Lund University Research Groups,Metabolic disorders and liver disease,A*Star Institute of Molecular and Cell Biology (IMCB),National University of Singapore(Swepub:lu)ph6235ka (author)
  • A*Star Institute of Molecular and Cell Biology (IMCB)A*Star, Bioinformatics Institute (BII) (creator_code:org_t)

Related titles

  • In:Cellular and Molecular Life Sciences: Springer Science and Business Media LLC1420-682X1420-9071

Internet link

Find in a library

To the university's database

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view