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Implementation of a...
Implementation of a protein profiling platform developed as an academic-pharmaceutical industry collaborative effort
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- Végvári, Akos (författare)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH
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- Magnusson, Mattias (författare)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH
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- Wallman, Lars (författare)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Elektroteknik (CI),Utbildningsprogram, LTH,Neuronano Research Center (NRC),Forskargrupper vid Lunds universitet,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,Electrical Engineering (M.Sc.Eng.),Educational programmes, LTH,Faculty of Engineering, LTH,Lund University Research Groups
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- Ekström, Simon (författare)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,BioMS,Forskargrupper vid Lunds universitet,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,Lund University Research Groups
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- Bolmsjö, Gunnar (författare)
- Lund University,Lunds universitet,Innovation,Institutionen för designvetenskaper,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Design Sciences,Departments at LTH,Faculty of Engineering, LTH
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- Nilsson, Johan (författare)
- Lund University,Lunds universitet,Byggnadsmekanik,Institutionen för byggvetenskaper,Institutioner vid LTH,Lunds Tekniska Högskola,Structural Mechanics,Department of Construction Sciences,Departments at LTH,Faculty of Engineering, LTH,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Department of Biomedical Engineering,Faculty of Engineering, LTH
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- Miliotis, Tasso (författare)
- AstraZeneca, Sweden
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- Ostling, Jörgen (författare)
- AstraZeneca, Sweden
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- Kjellström, Sven (författare)
- AstraZeneca, Sweden
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- Ottervald, Jan (författare)
- AstraZeneca, Sweden
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- Franzén, Bo (författare)
- Karolinska Institutet
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- Hultberg, Hans (författare)
- AstraZeneca, Sweden
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- Marko-Varga, György (författare)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Centrum för analys och syntes,Kemiska institutionen,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,Centre for Analysis and Synthesis,Department of Chemistry,Faculty of Engineering, LTH
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- Laurell, Thomas (författare)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,SEBRA Sepsis and Bacterial Resistance Alliance,Forskargrupper vid Lunds universitet,Masspektrometri,Sektion V,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Acoustofluidics group,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,Lund University Research Groups,Mass Spectrometry,Section V,Department of Clinical Sciences, Lund,Faculty of Medicine
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(creator_code:org_t)
- Wiley, 2008
- 2008
- Engelska 10 s.
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Ingår i: Electrophoresis. - : Wiley. - 0173-0835 .- 1522-2683. ; 29:12, s. 705-2696
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Abstract
Ämnesord
Stäng
- As much attention has devoted to the proteome research during the last few years, biomarker discovery has become an increasingly hot area, potentially enabling the development of new assays for diagnosis and prognosis of severe diseases. This is the field of research interest where efforts originating from both academic and industrial groups should jointly work on solutions. In this paper, we would like to demonstrate the fruitful combination of both research domains where the scientific crossroads sprout fresh ideas from the basic research domain and how these are refined and tethered to industrial standards. We will present an approach that is based on novel microfluidic devices, utilizing their benefits in processing small-volume samples. Our biomarker discovery strategy, built around this platform, involves optimized samples processing (based on SPE and sample enrichment) and fast MALDI-MS readout. The identification of novel biomarkers at low-abundance level has been achieved by the utilization of a miniaturized sample handling platform, which offers clean-up and enrichment of proteins in one step. Complete automation has been realized in the form of a unique robotic instrumentation that is able to extract and transfer 96 samples onto standard MALDI target plates with high throughput. The developed platform was operated with a 60 sample turnaround per hour allowing sensitivities in femtomol regions of medium- and low-abundant target proteins from clinical studies on samples of multiple sclerosis and gastroesophageal reflux disease. Several proteins have been identified as new biomarkers from cerebrospinal fluid and esophagus epithelial cells.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering (hsv//eng)
Nyckelord
- Academies and Institutes
- Biomarkers/metabolism
- Biomedical Research/organization & administration
- Cooperative Behavior
- Drug Industry
- Electrophoresis, Gel, Two-Dimensional
- Epithelial Cells/metabolism
- Esophagus/metabolism
- Gastroesophageal Reflux/metabolism
- Humans
- Microfluidic Analytical Techniques
- Multiple Sclerosis/metabolism
- Proteome/metabolism
- Robotics
- Solid Phase Extraction
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Tandem Mass Spectrometry
- microfluidics
- solid-phase extraction
- multiple sclerosis
- piezo-dispensing
- gastroesophageal reflux disease
- proteomics
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Végvári, Akos
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Magnusson, Matti ...
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Wallman, Lars
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Ekström, Simon
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Bolmsjö, Gunnar
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Nilsson, Johan
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visa fler...
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Miliotis, Tasso
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Ostling, Jörgen
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Kjellström, Sven
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Ottervald, Jan
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Franzén, Bo
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Hultberg, Hans
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Marko-Varga, Gyö ...
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Laurell, Thomas
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
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och Analytisk kemi
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- TEKNIK OCH TEKNOLOGIER
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TEKNIK OCH TEKNO ...
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och Medicinteknik
- Artiklar i publikationen
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Electrophoresis
- Av lärosätet
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Lunds universitet
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Karolinska Institutet