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Search: id:"swepub:oai:lup.lub.lu.se:058c31f0-2d9e-4676-a1eb-7f0c90ce6ea5" > Decellularization a...

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  • Mayorca-Guiliani, Alejandro EUniversity of Copenhagen (author)

Decellularization and antibody staining of mouse tissues to map native extracellular matrix structures in 3D

  • Article/chapterEnglish2019

Publisher, publication year, extent ...

  • 2019-11-08
  • Springer Science and Business Media LLC,2019

Numbers

  • LIBRIS-ID:oai:lup.lub.lu.se:058c31f0-2d9e-4676-a1eb-7f0c90ce6ea5
  • https://lup.lub.lu.se/record/058c31f0-2d9e-4676-a1eb-7f0c90ce6ea5URI
  • https://doi.org/10.1038/s41596-019-0225-8DOI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:art swepub-publicationtype
  • Subject category:ref swepub-contenttype

Notes

  • The extracellular matrix (ECM) is a major regulator of homeostasis and disease, yet the 3D structure of the ECM remains poorly understood because of limitations in ECM visualization. We recently developed an ECM-specialized method termed in situ decellularization of tissues (ISDoT) to isolate native 3D ECM scaffolds from whole organs in which ECM structure and composition are preserved. Here, we present detailed surgical instructions to facilitate decellularization of 33 different mouse tissues and details of validated antibodies that enable the visualization of 35 mouse ECM proteins. Through mapping of these ECM proteins, the structure of the ECM can be determined and tissue structures visualized in detail. In this study, perfusion decellularization is presented for bones, skeletal muscle, tongue, salivary glands, stomach, duodenum, jejunum/ileum, large intestines, mesentery, liver, gallbladder, pancreas, trachea, bronchi, lungs, kidneys, urinary bladder, ovaries, uterine horn, cervix, adrenal gland, heart, arteries, veins, capillaries, lymph nodes, spleen, peripheral nerves, eye, outer ear, mammary glands, skin, and subcutaneous tissue. Decellularization, immunostaining, and imaging take 4-5 d.

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  • Willacy, OliverUniversity of Copenhagen (author)
  • Madsen, Chris DLund University,Lunds universitet,Avdelningen för translationell cancerforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Cancer and matrix remodelling,Forskargrupper vid Lunds universitet,Division of Translational Cancer Research,Department of Laboratory Medicine,Faculty of Medicine,Lund University Research Groups(Swepub:lu)ch3375ma (author)
  • Rafaeva, MariaUniversity of Copenhagen (author)
  • Elisabeth Heumüller, StefanieUniversity of Cologne (author)
  • Bock, FelixUniversity Hospital of Cologne (author)
  • Sengle, GerhardUniversity Hospital of Cologne,University of Cologne (author)
  • Koch, ManuelUniversity Hospital of Cologne (author)
  • Imhof, ThomasUniversity Hospital of Cologne (author)
  • Zaucke, FrankOrthopaedic University Hospital Friedrichsheim (author)
  • Wagener, RaimundUniversity of Cologne (author)
  • Sasaki, TakakoOita University (author)
  • Erler, Janine TUniversity of Copenhagen (author)
  • Reuten, RaphaelUniversity of Copenhagen (author)
  • University of CopenhagenAvdelningen för translationell cancerforskning (creator_code:org_t)

Related titles

  • In:Nature Protocols: Springer Science and Business Media LLC14, s. 3395-34251750-27991754-2189

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