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Aeration rate effec...
Aeration rate effect on the growth kinetics, phytase production and plasmid stability of recombinant Escherichia coli BL21 (DE3)
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- Othman, Nor Zalina (author)
- University of Technology Malaysia (UTM)
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- Elsayed, Elsayed A. (author)
- King Saud University,Nuclear Research Center (NRC)
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- Malek, Roslinda Abd (author)
- University of Technology Malaysia (UTM)
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- Ramli, Solleh (author)
- University of Technology Malaysia (UTM)
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- Masri, Helmy J. (author)
- University of Technology Malaysia (UTM)
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- Sarmidi, Mohamed R. (author)
- University of Technology Malaysia (UTM)
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- Aziz, Ramlan (author)
- University of Technology Malaysia (UTM)
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- Wadaan, Mohamad A. (author)
- King Saud University
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- Hatti-Kaul, Rajni (author)
- Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- El Enshasy, Hesham A. (author)
- University of Technology Malaysia (UTM),Skåne University Hospital
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(creator_code:org_t)
- 2014
- 2014
- English 8 s.
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In: Journal of Pure and Applied Microbiology. - 0973-7510. ; 8:4, s. 2721-2728
- Related links:
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https://lup.lub.lu.s...
Abstract
Subject headings
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- Phytase production using Escherichia coli BL21 (DE3), a recombinant strain harboring a plasmid encoding thermostable Bacillus phytase, in semi-industrial scale was studied in this work. Among the factors needed to be considered in order to achieve high enzyme yield, aeration rate plays an important role. Suitable aeration is required to supply cells with sufficient amount of air for cell and phytase production. The effect of different aeration rates, (1.0, 2.0 and 3.0 vv-1min-1), on the kinetics of cell growth and phytase production by the recombinant E. coli BL21 (DE3) in 16-L pilot scale stirred tank bioreactor was investigated. The highest cell concentration of 3.81 gL-1 concomitant with the maximal total phytase production of 15.63 UmL-1 were obtained in a bioreactor of aeration rate 3.0vv-1min-1. At this high aeration rate, a maximal specific growth rate (μmax) and the maximal specific phytase production of 0.33 h-1 and 4102Ug-1, respectively, were achieved.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
Keyword
- Aeration rate
- Bioreactor cultivation
- Escherichia coli
- Phytase production
- Plasmid stability
Publication and Content Type
- art (subject category)
- ref (subject category)
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Othman, Nor Zali ...
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Elsayed, Elsayed ...
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Malek, Roslinda ...
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Ramli, Solleh
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Masri, Helmy J.
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Sarmidi, Mohamed ...
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Aziz, Ramlan
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Wadaan, Mohamad ...
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Hatti-Kaul, Rajn ...
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El Enshasy, Hesh ...
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Industrial Biote ...
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Journal of Pure ...
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Lund University