Probing the coverage of nanoparticles by biomimetic membranes through nanoplasmonics

Although promising for biomedicine, the clinical translation of inorganic nanoparticles (NPs) is limited by low biocompatibility and stability in biological fluids. A common strategy to circumvent this drawback consists in disguising the active inorganic core with a lipid bilayer coating, reminiscent of the structure of the cell membrane to redefine the chemical and biological identity of NPs. While recent reports introduced membrane-coating procedures for NPs, a robust and accessible method to quantify the integrity of the bilayer coverage is not yet available. To fill this gap, we prepared SiO2 nanoparticles (SiO2NPs) with different membrane coverage degrees and monitored their interaction with AuNPs by combining microscopic, scattering, and optical techniques. The membrane-coating on SiO2NPs induces spontaneous clustering of AuNPs, whose extent depends on the coating integrity. Remarkably, we discovered a linear correlation between the membrane coverage and a spectral descriptor for the AuNPs’ plasmonic resonance, spanning a wide range of coating yields. These results provide a fast and cost-effective assay to monitor the compatibilization of NPs with biological environments, essential for bench tests and scale-up. In addition, we introduce a robust and scalable method to prepare SiO2NPs/AuNPs hybrids through spontaneous self-assembly, with a high-fidelity structural control mediated by a lipid bilayer.

TEKNIK OCH TEKNOLOGIER Nanoteknik hsv//swe
ENGINEERING AND TECHNOLOGY Nano-technology hsv//eng
NATURVETENSKAP Fysik Atom- och molekylfysik och optik hsv//swe
NATURAL SCIENCES Physical Sciences Atom and Molecular Physics and Optics hsv//eng
Biomimetic nanoparticles
Extracellular vesicles
Gold nanoparticles
Membrane-coated nanoparticles
Nano-bio interface
Nanomedicine
Nanoplasmonics
Silica nanoparticles

Ridolfi, AndreaCNR Institute of nanostructured materials (ISMN-CNR),University of Florence,Vrije Universiteit Amsterdam (författare)
Donati, MelissaUniversity of Florence (författare)
Giampietro, ValentinaUniversity of Florence (författare)
Severi, MirkoUniversity of Florence (författare)
Brucale, MarcoUniversity of Florence,CNR Institute of nanostructured materials (ISMN-CNR) (författare)
Valle, FrancescoCNR Institute of nanostructured materials (ISMN-CNR),University of Florence (författare)
Bergese, PaoloUniversity of Brescia,University of Florence (författare)
Montis, CostanzaUniversity of Florence (författare)
Caselli, LucreziaLund University,Lunds universitet,Fysikalisk kemi,Enheten för fysikalisk och teoretisk kemi,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Physical Chemistry,Physical and theoretical chemistry,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,University of Florence(Swepub:lu)lu7016ca (författare)
Berti, DeboraUniversity of Florence (författare)
University of FlorenceCNR Institute of nanostructured materials (ISMN-CNR) (creator_code:org_t)

Ingår i:Journal of Colloid and Interface Science: Elsevier BV640, s. 100-1090021-9797

Av författaren/redakt...: Cardellini, Jaco ...; Ridolfi, Andrea; Donati, Melissa; Giampietro, Vale ...; Severi, Mirko; Brucale, Marco; visa fler...; Valle, Francesco; Bergese, Paolo; Montis, Costanza; Caselli, Lucrezi ...; Berti, Debora; visa färre...

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