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Injury-related cell...
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Kosonen, Joonas P.University of Eastern Finland
(författare)
Injury-related cell death and proteoglycan loss in articular cartilage : Numerical model combining necrosis, reactive oxygen species, and inflammatory cytokines
- Artikel/kapitelEngelska2023
Förlag, utgivningsår, omfång ...
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2023-01-26
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Public Library of Science (PLoS),2023
Nummerbeteckningar
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LIBRIS-ID:oai:lup.lub.lu.se:08e36d77-72c6-4259-a020-7064b6ed75ec
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https://lup.lub.lu.se/record/08e36d77-72c6-4259-a020-7064b6ed75ecURI
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https://doi.org/10.1371/journal.pcbi.1010337DOI
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Språk:engelska
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Sammanfattning på:engelska
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Klassifikation
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Ämneskategori:art swepub-publicationtype
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Ämneskategori:ref swepub-contenttype
Anmärkningar
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Osteoarthritis (OA) is a common musculoskeletal disease that leads to deterioration of articular cartilage, joint pain, and decreased quality of life. When OA develops after a joint injury, it is designated as post-traumatic OA (PTOA). The etiology of PTOA remains poorly understood, but it is known that proteoglycan (PG) loss, cell dysfunction, and cell death in cartilage are among the first signs of the disease. These processes, influenced by biomechanical and inflammatory stimuli, disturb the normal cell-regulated balance between tissue synthesis and degeneration. Previous computational mechanobiological models have not explicitly incorporated the cell-mediated degradation mechanisms triggered by an injury that eventually can lead to tissue-level compositional changes. Here, we developed a 2-D mechanobiological finite element model to predict necrosis, apoptosis following excessive production of reactive oxygen species (ROS), and inflammatory cytokine (interleukin-1)-driven apoptosis in cartilage explant. The resulting PG loss over 30 days was simulated. Biomechanically triggered PG degeneration, associated with cell necrosis, excessive ROS production, and cell apoptosis, was predicted to be localized near a lesion, while interleukin-1 diffusion-driven PG degeneration was manifested more globally. Interestingly, the model also showed proteolytic activity and PG biosynthesis closer to the levels of healthy tissue when pro-inflammatory cytokines were rapidly inhibited or cleared from the culture medium, leading to partial recovery of PG content. The numerical predictions of cell death and PG loss were supported by previous experimental findings. Furthermore, the simulated ROS and inflammation mechanisms had longer-lasting effects (over 3 days) on the PG content than localized necrosis. The mechanobiological model presented here may serve as a numerical tool for assessing early cartilage degeneration mechanisms and the efficacy of interventions to mitigate PTOA progression.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Eskelinen, Atte S.A.University of Eastern Finland
(författare)
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Orozco, Gustavo A.Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,LTH profilområde: Teknik för hälsa,LTH profilområden,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,LTH Profile Area: Engineering Health,LTH Profile areas,Faculty of Engineering, LTH,University of Eastern Finland(Swepub:lu)gu0063or
(författare)
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Nieminen, PetteriUniversity of Eastern Finland
(författare)
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Anderson, Donald D.University of Iowa
(författare)
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Grodzinsky, Alan J.Massachusetts Institute of Technology
(författare)
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Korhonen, Rami K.University of Eastern Finland
(författare)
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Tanska, PetriUniversity of Eastern Finland
(författare)
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University of Eastern FinlandAvdelningen för Biomedicinsk teknik
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:PLoS Computational Biology: Public Library of Science (PLoS)19:11553-734X1553-7358
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