Simultaneous Measurements of Dinitrogen Fixation and Denitrification Associated With Coral Reef Substrates: Advantages and Limitations of a Combined Acetylene Assay

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: (WFRF:(Voolstra Christian R.)) > (2020-2022) > Simultaneous Measur...

Details
MARC

El-Khaledl, Yusuf C. (author)

Simultaneous Measurements of Dinitrogen Fixation and Denitrification Associated With Coral Reef Substrates : Advantages and Limitations of a Combined Acetylene Assay

Article/chapterEnglish2020

Publisher, publication year, extent ...

2020-06-11
Frontiers Media SA,2020
printrdacarrier

Numbers

LIBRIS-ID:oai:DiVA.org:su-183640
https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-183640URI
https://doi.org/10.3389/fmars.2020.00411DOI

Supplementary language notes

Language:English
Summary in:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

Nitrogen (N) cycling in coral reefs is of key importance for these oligotrophic ecosystems, but knowledge about its pathways is limited. While dinitrogen (N-2) fixation is comparably well studied, the counteracting denitrification pathway is under-investigated, mainly because of expensive and relatively complex experimental techniques currently available. Here, we combined two established acetylene-based assays to one single setup to determine N-2-fixation and denitrification performed by microbes associated with coral reef substrates/organisms simultaneously. Accumulating target gases (ethylene for N-2-fixation, nitrous oxide for denitrification) were measured in gaseous headspace samples via gas chromatography. We measured N-2-fixation and denitrification rates of two Red Sea coral reef substrates (filamentous turf algae, coral rubble), and demonstrated, for the first time, the co-occurrence of both N-cycling processes in both substrates. N-2-fixation rates were up to eight times higher during the light compared to the dark, whereas denitrification rates during dark incubations were stimulated for turf algae and suppressed for coral rubble compared to light incubations. Our results highlight the importance of both substrates in fixing N, but their role in relieving N is potentially divergent. Absolute N-2-fixation rates of the present study correspond with rates reported previously, even though likely underestimated due to an initial lag phase. Denitrification is also presumably underestimated due to incomplete nitrous oxide inhibition and/or substrate limitation. Besides these inherent limitations, we show that a relative comparison of N-2-fixation and denitrification activity between functional groups is possible. Thus, our approach facilitates cost-efficient sample processing in studies interested in comparing relative rates of N-2-fixation and denitrification.

Subject headings and genre

NATURVETENSKAP Geovetenskap och miljövetenskap hsv//swe
NATURAL SCIENCES Earth and Related Environmental Sciences hsv//eng
NATURVETENSKAP Biologi hsv//swe
NATURAL SCIENCES Biological Sciences hsv//eng
nitrogen cycling
metabolism
gas chromatography
ethylene
nitrous oxide

Added entries (persons, corporate bodies, meetings, titles ...)

Roth, FlorianStockholms universitet,Stockholms universitets Östersjöcentrum,King Abdullah University of Science and Technology, Saudi Arabia; University of Helsinki, Finland(Swepub:su)flro3057 (author)
Rädecker, Nils (author)
Kharbatia, Najeh (author)
Jones, Burton H. (author)
Voolstra, Christian R. (author)
Wild, Christian (author)
Stockholms universitetStockholms universitets Östersjöcentrum (creator_code:org_t)

Related titles

In:Frontiers in Marine Science: Frontiers Media SA72296-7745

Internet link

Find in a library

Frontiers in Marine Science (Search for host publication in LIBRIS)

To the university's database

Find more in SwePub

By the author/editor: El-Khaledl, Yusu ...; Roth, Florian; Rädecker, Nils; Kharbatia, Najeh; Jones, Burton H.; Voolstra, Christ ...; show more...; Wild, Christian; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Earth and Relate ...

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...

Articles in the publication: Frontiers in Mar ...

By the university: Stockholm University

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se