Sökning: id:"swepub:oai:lup.lub.lu.se:0e344b7e-9334-4856-8d16-50f3d98d8329" >
The pentatricopepti...
-
Fan, KaijianChinese Academy of Agricultural Sciences
(författare)
The pentatricopeptide repeat protein EMP603 is required for the splicing of mitochondrial Nad1 intron 2 and seed development in maize
- Artikel/kapitelEngelska2021
Förlag, utgivningsår, omfång ...
-
2021-07-19
-
Oxford University Press (OUP),2021
-
16 s.
Nummerbeteckningar
-
LIBRIS-ID:oai:lup.lub.lu.se:0e344b7e-9334-4856-8d16-50f3d98d8329
-
https://lup.lub.lu.se/record/0e344b7e-9334-4856-8d16-50f3d98d8329URI
-
https://doi.org/10.1093/jxb/erab339DOI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:art swepub-publicationtype
-
Ämneskategori:ref swepub-contenttype
Anmärkningar
-
Intron splicing is an essential event in post-transcriptional RNA processing in plant mitochondria, which requires the participation of diverse nuclear-encoded splicing factors. However, it is presently unclear how these proteins cooperatively take part in the splicing of specific introns. In this study, we characterized a nuclear-encoded mitochondrial P-type pentatricopeptide repeat (PPR) protein named EMP603. This protein is essential for splicing of intron 2 in the Nad1 gene and interacts with the mitochondria-localized DEAD-box RNA helicase PMH2-5140, the RAD52-like proteins ODB1-0814 and ODB1-5061, and the CRM domain-containing protein Zm-mCSF1. Further study revealed that the N-terminal region of EMP603 interacts with the DEAD-box of PMH2-5140, the CRM domain of Zm-mCSF1, and OBD1-5061, but not with OBD1-0814, whereas the PPR domain of EMP603 can interact with ODB1-0814, ODB1-5061, and PMH2-5140, but not with Zm-mCSF1. Defects in EMP603 severely disrupt the assembly and activity of mitochondrial complex I, leading to impaired mitochondrial function, and delayed seed development. The interactions revealed between EMP603 and PMH2-5140, ODB1-0814, ODB1-5061, and Zm-mCSF1 indicate a possible involvement of a dynamic 'spliceosome-like' complex in intron splicing, and may accelerate the elucidation of the intron splicing mechanism in plant mitochondria.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Ren, ZhenjingChinese Academy of Agricultural Sciences
(författare)
-
Zhang, XiaofengChinese Academy of Agricultural Sciences
(författare)
-
Liu, YanChinese Academy of Agricultural Sciences
(författare)
-
Fu, JunjieChinese Academy of Agricultural Sciences
(författare)
-
Qi, ChunlaiChinese Academy of Agricultural Sciences
(författare)
-
Tatar, WurinileChinese Academy of Agricultural Sciences
(författare)
-
Rasmusson, Allan G.Lund University,Lunds universitet,Molekylär cellbiologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Växtbiologi,Forskargrupper vid Lunds universitet,Molecular Cell Biology,Department of Biology,Faculty of Science,Plant Biology,Lund University Research Groups(Swepub:lu)fysb-ara
(författare)
-
Wang, GuoyingChinese Academy of Agricultural Sciences
(författare)
-
Liu, YunjunChinese Academy of Agricultural Sciences
(författare)
-
Chinese Academy of Agricultural SciencesMolekylär cellbiologi
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:Journal of Experimental Botany: Oxford University Press (OUP)72:20, s. 6933-69480022-09571460-2431
Internetlänk
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Fan, Kaijian
-
Ren, Zhenjing
-
Zhang, Xiaofeng
-
Liu, Yan
-
Fu, Junjie
-
Qi, Chunlai
-
visa fler...
-
Tatar, Wurinile
-
Rasmusson, Allan ...
-
Wang, Guoying
-
Liu, Yunjun
-
visa färre...
- Om ämnet
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Biokemi och mole ...
- Artiklar i publikationen
-
Journal of Exper ...
- Av lärosätet
-
Lunds universitet