The pentatricopeptide repeat protein EMP603 is required for the splicing of mitochondrial Nad1 intron 2 and seed development in maize

Intron splicing is an essential event in post-transcriptional RNA processing in plant mitochondria, which requires the participation of diverse nuclear-encoded splicing factors. However, it is presently unclear how these proteins cooperatively take part in the splicing of specific introns. In this study, we characterized a nuclear-encoded mitochondrial P-type pentatricopeptide repeat (PPR) protein named EMP603. This protein is essential for splicing of intron 2 in the Nad1 gene and interacts with the mitochondria-localized DEAD-box RNA helicase PMH2-5140, the RAD52-like proteins ODB1-0814 and ODB1-5061, and the CRM domain-containing protein Zm-mCSF1. Further study revealed that the N-terminal region of EMP603 interacts with the DEAD-box of PMH2-5140, the CRM domain of Zm-mCSF1, and OBD1-5061, but not with OBD1-0814, whereas the PPR domain of EMP603 can interact with ODB1-0814, ODB1-5061, and PMH2-5140, but not with Zm-mCSF1. Defects in EMP603 severely disrupt the assembly and activity of mitochondrial complex I, leading to impaired mitochondrial function, and delayed seed development. The interactions revealed between EMP603 and PMH2-5140, ODB1-0814, ODB1-5061, and Zm-mCSF1 indicate a possible involvement of a dynamic 'spliceosome-like' complex in intron splicing, and may accelerate the elucidation of the intron splicing mechanism in plant mitochondria.

Ren, ZhenjingChinese Academy of Agricultural Sciences (författare)
Zhang, XiaofengChinese Academy of Agricultural Sciences (författare)
Liu, YanChinese Academy of Agricultural Sciences (författare)
Fu, JunjieChinese Academy of Agricultural Sciences (författare)
Qi, ChunlaiChinese Academy of Agricultural Sciences (författare)
Tatar, WurinileChinese Academy of Agricultural Sciences (författare)
Rasmusson, Allan G.Lund University,Lunds universitet,Molekylär cellbiologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Växtbiologi,Forskargrupper vid Lunds universitet,Molecular Cell Biology,Department of Biology,Faculty of Science,Plant Biology,Lund University Research Groups(Swepub:lu)fysb-ara (författare)
Wang, GuoyingChinese Academy of Agricultural Sciences (författare)
Liu, YunjunChinese Academy of Agricultural Sciences (författare)
Chinese Academy of Agricultural SciencesMolekylär cellbiologi (creator_code:org_t)

Ingår i:Journal of Experimental Botany: Oxford University Press (OUP)72:20, s. 6933-69480022-09571460-2431

Av författaren/redakt...: Fan, Kaijian; Ren, Zhenjing; Zhang, Xiaofeng; Liu, Yan; Fu, Junjie; Qi, Chunlai; visa fler...; Tatar, Wurinile; Rasmusson, Allan ...; Wang, Guoying; Liu, Yunjun; visa färre...

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